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  • Featured Product
  • KD/KO Validated

BCL2 Monoclonal antibody, PBS Only

BCL2 Monoclonal Antibody for IP, IF, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

IP, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4H8C6

Cat no : 60178-1-PBS

Synonyms

Apoptosis regulator Bcl 2, B cell CLL/lymphoma 2, BCL2, BCL-2, Oncogene B cell leukemia 2

Validation Data Gallery

Applications:
  • IP experiment of U-937 using 60178-1-Ig (same clone as 60178-1-PBS)

    IP experiment of U-937 using 60178-1-Ig (same clone as 60178-1-PBS)

    IP result of anti-BCL2 (IP:60178-1-Ig, 5ug; Detection:60178-1-Ig 1:1000) with U-937 cells lysate 4000ug. This data was developed using the same antibody clone with 60178-1-PBS in a different storage buffer formulation.

  • IHC staining of human appendicitis using 60178-1-Ig (same clone as 60178-1-PBS)

    IHC staining of human appendicitis using 60178-1-Ig (same clone as 60178-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60178-1-Ig (BCL2 antibody) at dilution of 1:12800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60178-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BCL2 antibody included
  • IHC staining of human appendicitis using 60178-1-Ig (same clone as 60178-1-PBS)

    IHC staining of human appendicitis using 60178-1-Ig (same clone as 60178-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60178-1-Ig (BCL2 antibody) at dilution of 1:12800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60178-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BCL2 antibody included
  • IHC staining of Jurkat using 60178-1-Ig (same clone as 60178-1-PBS)

    IHC staining of Jurkat using 60178-1-Ig (same clone as 60178-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 60178-1-Ig (BCL2 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60178-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BCL2 antibody included
  • IHC staining of human lymphoma using 60178-1-Ig (same clone as 60178-1-PBS)

    IHC staining of human lymphoma using 60178-1-Ig (same clone as 60178-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60178-1-Ig (BCL2 antibody) at dilution of 1:12800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60178-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BCL2 antibody included
  • IHC staining of human lymphoma using 60178-1-Ig (same clone as 60178-1-PBS)

    IHC staining of human lymphoma using 60178-1-Ig (same clone as 60178-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60178-1-Ig (BCL2 antibody) at dilution of 1:12800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60178-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BCL2 antibody included
  • IHC staining of human tonsillitis using 60178-1-Ig (same clone as 60178-1-PBS)

    IHC staining of human tonsillitis using 60178-1-Ig (same clone as 60178-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60178-1-Ig (BCL2 Antibody) at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 60178-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BCL2 antibody included
  • IHC staining of human tonsillitis using 60178-1-Ig (same clone as 60178-1-PBS)

    IHC staining of human tonsillitis using 60178-1-Ig (same clone as 60178-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60178-1-Ig (BCL2 Antibody) at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 60178-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BCL2 antibody included
  • IF Staining of MCF-7 using 60178-1-Ig (same clone as 60178-1-PBS)

    IF Staining of MCF-7 using 60178-1-Ig (same clone as 60178-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using BCL2 antibody (60178-1-Ig, Clone: 4H8C6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60178-1-PBS in a different storage buffer formulation.

  • IF Staining of human appendicitis using 60178-1-Ig (same clone as 60178-1-PBS)

    IF Staining of human appendicitis using 60178-1-Ig (same clone as 60178-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using BCL2 antibody (60178-1-Ig, Clone: 4H8C6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60178-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 60178-1-Ig (same clone as 60178-1-PBS)

    IF Staining of human tonsillitis using 60178-1-Ig (same clone as 60178-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using BCL2 antibody (60178-1-Ig, Clone: 4H8C6 ) at dilution of 1:1500 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60178-1-PBS in a different storage buffer formulation.

  • FC experiment of human peripheral blood lymphocytes using 60178-1-Ig (same clone as 60178-1-PBS)

    FC experiment of human peripheral blood lymphocytes using 60178-1-Ig (same clone as 60178-1-PBS)

    1X10^6 human peripheral blood lymphocytes were stained with 0.2 ug Anti-Human BCL2 (60178-1-Ig, Clone:4H8C6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or 0.2 ug isotype control and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. This data was developed using the same antibody clone with 60178-1-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 60178-1-Ig (same clone as 60178-1-PBS)

    FC experiment of Jurkat using 60178-1-Ig (same clone as 60178-1-PBS)

    1X10^6 Jurkat cells were stained with 0.2ug BCL2 antibody (60178-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. This data was developed using the same antibody clone with 60178-1-PBS in a different storage buffer formulation.

Tested Applications

Planning an IHC experiment? We recommend our IHCeasy BCL2 Ready-To-Use IHC Kit. BCL2 primary antibody included.

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

60178-1-PBS targets BCL2 in IP, IF, FC, IHC, Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen BCL2 fusion protein Ag3508 相同性解析による交差性が予測される生物種
Full Name B-cell CLL/lymphoma 2
Calculated molecular weight 26 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC027258
Gene symbol BCL2
Gene ID (NCBI) 596
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

1) What is Bcl-2?
Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia.
2) FAQs for Bcl-2

A) How to measure Bcl-2 and Bax apoptotic markers by western blotting
Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.
B) What band size should I expect in western blotting for Bcl-2?
The molecular weight of Bcl-2 is 26 kDa.