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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using CoraLite®594-conjugated p130Cas / BCAR1 Monoclonal (CL594-67215)

    IF Staining of human breast cancer using CL594-67215

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594 BCAR1 antibody (CL594-67215, Clone: 2D11C10 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite®594-conjugated p130Cas / BCAR1 Monoclonal (CL594-67215)

    IF Staining of MCF-7 using CL594-67215

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite®594 p130Cas / BCAR1 antibody (CL594-67215, Clone: 2D11C10 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of A431 cells using CoraLite®594-conjugated p130Cas / BCAR1 Monoclonal (CL594-67215)

    FC experiment of A431 using CL594-67215

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human p130Cas / BCAR1 (CL594-67215, Clone:2D11C10) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

CoraLite®594-conjugated p130Cas / BCAR1 Monoclonal antibody

p130Cas / BCAR1 Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)
Cat No. CL594-67215
Clone No.2D11C10

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC, IF-P, FC (Intra)

Synonyms

BCAR1, 2D11C10, Breast cancer anti-estrogen resistance protein 1, CAS, CAS1

Formulation:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF-P detected inhuman breast cancer tissue
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67215 targets p130Cas / BCAR1 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag10604

Product name: Recombinant human BCAR1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-420 aa of BC062556

Sequence: MNHLNVLAKALYDNVAESPDELSFRKGDIMTVLEQDTQGLDGWWLCSLHGRQGIVPGNRLKILVGMYDKKPAGPGSGPPATPAQPQPGLHAPAPPASQYTPMLPNTYQPQPDSVYLVPTPSKAQQGLYQVPGPSPQFQSPPAKQTSTFSKQTPHHPFPNPATDLYQVPPGPGGPAQDIYQVPPSAGMGHDIYQVPPSMDTRSWEGTKPPAKVVVPTRVGQGYVYEAAQPEQDEYDIPRHLLAPGPQDIYDVPPVRGLLPSQYGQEVYDTPPMAVKGPNGRDPLLEVYDVPPSVEKGLPPSNHHAVYDVPPSVSKDVPDGPLLREETYDVPPAFAKAKPFDPARTPLVLAAPPPDSPPAEDVYDVPPPAPDLYDVPPGLRRPGPGTLYDVPRERVLPPEVADGGVVDSGVYAVPPPAEREA

相同性解析による交差性が予測される生物種
Full Name breast cancer anti-estrogen resistance 1
Calculated molecular weight 870 aa, 93 kDa
GenBank accession numberBC062556
Gene Symbol BCAR1
Gene ID (NCBI) 9564
RRIDAB_2920097
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP56945
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
FC protocol for CL594 p130Cas / BCAR1 antibody CL594-67215Download protocol
IF protocol for CL594 p130Cas / BCAR1 antibody CL594-67215Download protocol
Standard Protocols
Click here to view our Standard Protocols
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