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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using B3GNT2 Polyclonal antibody (14200-1-AP)

    WB analysis using 14200-1-AP (same clone as 14200-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14200-1-AP (B3GNT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14200-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using B3GNT2 Polyclonal antibody (14200-1-AP)

    WB analysis using 14200-1-AP (same clone as 14200-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14200-1-AP (B3GNT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14200-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using B3GNT2 Polyclonal antibody (14200-1-AP)

    IHC staining of mouse testis using 14200-1-AP (same clone as 14200-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14200-1-AP (B3GNT2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14200-1-PBS in a different storage buffer formulation.

B3GNT2 Polyclonal antibody, PBS Only

B3GNT2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 14200-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

B3GALT7, b3Gal-T7, B3GNT1, Beta-1,3-galactosyltransferase 7, Beta-1,3-GalTase 7

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14200-1-PBS targets B3GNT2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5404

Product name: Recombinant human B3GNT2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 47-397 aa of BC030579

Sequence: KFWKISTPPEAYWNREQEKLNRQYNPILSMLTNQTGEAGRLSNISHLNYCEPDLRVTSVVTGFNNLPDRFKDFLLYLRCRNYSLLIDQPDKCAKKPFLLLAIKSLTPHFARRQAIRESWGQESNAGNQTVVRVFLLGQTPPEDNHPDLSDMLKFESEKHQDILMWNYRDTFFNLSLKEVLFLRWVSTSCPDTEFVFKGDDDVFVNTHHILNYLNSLSKTKAKDLFIGDVIHNAGPHRDKKLKYYIPEVVYSGLYPPYAGGGGFLYSGHLALRLYHITDQVHLYPIDDVYTGMCLQKLGLVPEKHKGFRTFDIEEKNKNNICSYVDLMLVHSRKPQEMIDIWSQLQSAHLKC

相同性解析による交差性が予測される生物種
Full Name UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2
Calculated molecular weight 45 kDa
Observed molecular weight45 kDa, 69 kDa
GenBank accession numberBC030579
Gene Symbol B3GNT2
Gene ID (NCBI) 10678
RRIDAB_3085431
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NY97
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

β-1,3-N-acetylglucosaminyltransferase (B3GNT2) is the most abundant and ubiquitously expressed B3GNT isoform and displays the greatest in vitro activity for N-glycan and O-glycan poly-LacNAc extensionis. B3GNT2 encodes a poly-N-acetyllactosamine synthase that targets >10 ligands and receptors to disrupt interactions between tumor and T cells and reduce T cell activation (PMID: 35338135, 32663509). B3GNT2 also has five predicted N-glycosylation sites at asparagine residues 79, 89, 127, 173, and 219 (PMID: 33229435). Western blots indicated the glycosylated form of B3GNT2 is detected at ~ 67 kDa.

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