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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using B3GNT2 Polyclonal antibody (14200-1-AP)

    WB analysis using 14200-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14200-1-AP (B3GNT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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B3GNT2 Polyclonal antibody

B3GNT2 Polyclonal Antibody for WB, ELISA
Cat No. 14200-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

b3Gal T7, B3GALT7, B3GN T1, B3GN T2, B3GNT, B3GNT 2, B3GNT1, B3GNT2, Beta 1,3 GalTase 7, Beta 1,3 Gn T1, Beta 1,3 Gn T2, Beta 3 Gx T7, Beta3Gal T7, Beta3GalT7, Beta3Gn T1, Beta3Gn T2, BETA3GNT, BGnT 1, BGnT 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, MCF-7 cells, SMMC-7721 cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14200-1-AP targets B3GNT2 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen B3GNT2 fusion protein Ag5404 相同性解析による交差性が予測される生物種
Full Name UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2
Calculated molecular weight 9 kDa
Observed molecular weight69 kDa
GenBank accession numberBC030579
Gene Symbol B3GNT2
Gene ID (NCBI) 10678
RRIDAB_3085431
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NY97
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

β-1,3-N-acetylglucosaminyltransferase (B3GNT2) is the most abundant and ubiquitously expressed B3GNT isoform and displays the greatest in vitro activity for N-glycan and O-glycan poly-LacNAc extensionis. B3GNT2 encodes a poly-N-acetyllactosamine synthase that targets >10 ligands and receptors to disrupt interactions between tumor and T cells and reduce T cell activation (PMID: 35338135, 32663509). B3GNT2 also has five predicted N-glycosylation sites at asparagine residues 79, 89, 127, 173, and 219 (PMID: 33229435). Western blots indicated the glycosylated form of B3GNT2 is detected at ~ 67 kDa.

Protocols

Product Specific Protocols
WB protocol for B3GNT2 antibody 14200-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols