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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Arfaptin-1 Monoclonal antibody (67461-1-Ig)

    WB analysis using 67461-1-Ig (same clone as 67461-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67461-1-Ig (ARFIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using Arfaptin-1 Monoclonal antibody (67461-1-Ig)

    WB analysis of HSC-T6 using 67461-1-Ig (same clone as 67461-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67461-1-Ig (ARFIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using Arfaptin-1 Monoclonal antibody (67461-1-Ig)

    WB analysis of NIH/3T3 using 67461-1-Ig (same clone as 67461-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67461-1-Ig (ARFIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of 4T1 cells using Arfaptin-1 Monoclonal antibody (67461-1-Ig)

    WB analysis of 4T1 using 67461-1-Ig (same clone as 67461-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 67461-1-Ig (ARFIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using Arfaptin-1 Monoclonal antibody (67461-1-Ig)

    IHC staining of mouse kidney using 67461-1-Ig (same clone as 67461-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67461-1-Ig (Arfaptin-1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using Arfaptin-1 Monoclonal antibody (67461-1-Ig)

    IHC staining of mouse kidney using 67461-1-Ig (same clone as 67461-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67461-1-Ig (Arfaptin-1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using Arfaptin-1 Monoclonal antibody (67461-1-Ig)

    IF Staining of mouse kidney using 67461-1-Ig (same clone as 67461-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Arfaptin-1 antibody (67461-1-Ig, Clone: 2A10B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using Arfaptin-1 Monoclonal antibody (67461-1-Ig)

    IF Staining of mouse kidney using 67461-1-Ig (same clone as 67461-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Arfaptin-1 antibody (67461-1-Ig, Clone: 2A10B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Arfaptin-1 Monoclonal antibody (67461-1-Ig)

    IF Staining of HeLa using 67461-1-Ig (same clone as 67461-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Arfaptin-1 antibody (67461-1-Ig, Clone: 2A10B3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using Arfaptin-1 Monoclonal antibody (67461-1-Ig)

    IF Staining of A549 using 67461-1-Ig (same clone as 67461-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Arfaptin-1 antibody (67461-1-Ig, Clone: 2A10B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51228-2

    Cytometric bead array standard curve of MP51228-2

    Cytometric bead array standard curve of MP51228-2, Arfaptin-1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67461-3-PBS. Detection antibody: 67461-1-PBS. Standard:Ag12097. Range: 3.125-100 ng/mL.

Arfaptin-1 Monoclonal antibody, PBS Only (Detector)

Arfaptin-1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 67461-1-PBS
Clone No.2A10B3

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Synonyms

ARFIP1, Arfaptin 1, ADP-ribosylation factor-interacting protein 1, 2A10B3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67461-1-PBS targets Arfaptin-1 as part of a matched antibody pair:

MP51228-2: 67461-3-PBS capture and 67461-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag12097

Product name: Recombinant human ARFIP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-373 aa of BC103759

Sequence: MAQESPKNSAAEIPVTSNGEVDDSREHSFNRDLKHSLPSGLGLSETQITSHGFDNTKEGVIEAGAFQGSPAPPLPSVMSPSRVAASRLAQQGSDLIVPAGGQRTQTKSGPVILADEIKNPAMEKLELVRKWSLNTYKCTRQIISEKLGRGSRTVDLELEAQIDILRDNKKKYENILKLAQTLSTQLFQMVHTQRQLGDAFADLSLKSLELHEEFGYNADTQKLLAKNGETLLGAINFFIASVNTLVNKTIEDTLMTVKQYESARIEYDAYRTDLEELNLGPRDANTLPKIEQSQHLFQAHKEKYDKMRNDVSVKLKFLEENKVKVLHNQLVLFHNAIAAYFAGNQKQLEQTLKQFHIKLKTPGVDAPSWLEEQ

相同性解析による交差性が予測される生物種
Full Name ADP-ribosylation factor interacting protein 1
Calculated molecular weight 373 aa, 42 kDa
Observed molecular weight 38-42 kDa
GenBank accession numberBC103759
Gene Symbol Arfaptin-1
Gene ID (NCBI) 27236
RRIDAB_2882691
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP53367
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.