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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Arfaptin-1 Monoclonal antibody (67461-1-Ig)

    WB analysis using 67461-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67461-1-Ig (ARFIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using Arfaptin-1 Monoclonal antibody (67461-1-Ig)

    WB analysis of HSC-T6 using 67461-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67461-1-Ig (ARFIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using Arfaptin-1 Monoclonal antibody (67461-1-Ig)

    WB analysis of NIH/3T3 using 67461-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67461-1-Ig (ARFIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of 4T1 cells using Arfaptin-1 Monoclonal antibody (67461-1-Ig)

    WB analysis of 4T1 using 67461-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 67461-1-Ig (ARFIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using Arfaptin-1 Monoclonal antibody (67461-1-Ig)

    IHC staining of mouse kidney using 67461-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67461-1-Ig (Arfaptin-1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using Arfaptin-1 Monoclonal antibody (67461-1-Ig)

    IHC staining of mouse kidney using 67461-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67461-1-Ig (Arfaptin-1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using Arfaptin-1 Monoclonal antibody (67461-1-Ig)

    IF Staining of mouse kidney using 67461-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Arfaptin-1 antibody (67461-1-Ig, Clone: 2A10B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using Arfaptin-1 Monoclonal antibody (67461-1-Ig)

    IF Staining of mouse kidney using 67461-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Arfaptin-1 antibody (67461-1-Ig, Clone: 2A10B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Arfaptin-1 Monoclonal antibody (67461-1-Ig)

    IF Staining of HeLa using 67461-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Arfaptin-1 antibody (67461-1-Ig, Clone: 2A10B3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using Arfaptin-1 Monoclonal antibody (67461-1-Ig)

    IF Staining of A549 using 67461-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Arfaptin-1 antibody (67461-1-Ig, Clone: 2A10B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Arfaptin-1 Monoclonal antibody

Arfaptin-1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 67461-1-Ig
Clone No.2A10B3

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

ARFIP1, Arfaptin 1, ADP-ribosylation factor-interacting protein 1, 2A10B3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, RAW 264.7 cells, HepG2 cells, Jurkat cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse kidney tissue
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67461-1-Ig targets Arfaptin-1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag12097

Product name: Recombinant human ARFIP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-373 aa of BC103759

Sequence: MAQESPKNSAAEIPVTSNGEVDDSREHSFNRDLKHSLPSGLGLSETQITSHGFDNTKEGVIEAGAFQGSPAPPLPSVMSPSRVAASRLAQQGSDLIVPAGGQRTQTKSGPVILADEIKNPAMEKLELVRKWSLNTYKCTRQIISEKLGRGSRTVDLELEAQIDILRDNKKKYENILKLAQTLSTQLFQMVHTQRQLGDAFADLSLKSLELHEEFGYNADTQKLLAKNGETLLGAINFFIASVNTLVNKTIEDTLMTVKQYESARIEYDAYRTDLEELNLGPRDANTLPKIEQSQHLFQAHKEKYDKMRNDVSVKLKFLEENKVKVLHNQLVLFHNAIAAYFAGNQKQLEQTLKQFHIKLKTPGVDAPSWLEEQ

相同性解析による交差性が予測される生物種
Full Name ADP-ribosylation factor interacting protein 1
Calculated molecular weight 373 aa, 42 kDa
Observed molecular weight 38-42 kDa
GenBank accession numberBC103759
Gene Symbol Arfaptin-1
Gene ID (NCBI) 27236
RRIDAB_2882691
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP53367
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Arfaptin-1 antibody 67461-1-IgDownload protocol
IHC protocol for Arfaptin-1 antibody 67461-1-IgDownload protocol
IF protocol for Arfaptin-1 antibody 67461-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols