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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Anti-DNA Recombinant antibody (85854-1-RR)

    IHC staining of human ovary tumor using 85854-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 85854-1-RR (Anti-DNA antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Anti-DNA Recombinant antibody (85854-1-RR)

    IHC staining of human tonsillitis using 85854-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 85854-1-RR (Anti-DNA antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using Anti-DNA Recombinant antibody (85854-1-RR)

    IHC staining of human placenta using 85854-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85854-1-RR (Anti-DNA antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using Anti-DNA Recombinant antibody (85854-1-RR)

    IHC staining of human placenta using 85854-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85854-1-RR (Anti-DNA antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HeLa cells using Anti-DNA Recombinant antibody (85854-1-RR)

    FC experiment of HeLa using 85854-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.2 ug N/a Anti-DNA Recombinant Antibody (85854-1-RR, Clone:250864A1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.2 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Dot Blot experiment using Anti-DNA Recombinant antibody (85854-1-RR)

    Dot Blot experiment using 85854-1-RR

    Dot blot analysis was used to confirm the specificity of 85854-1-RR anti-DNA antibody. samples were spotted onto NC and probed with antibody at 5 µg/ml.The amount of sample (μg) spotted is indicated next to each row.

DNA Recombinant monoclonal antibody

DNA Uni-rAb® Recombinant Antibody for IHC, FC (Intra), Dot Blot, ELISA
Cat No. 85854-1-RR
Clone No.250864A1
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

n/a

Applications

IHC, FC (Intra), Dot Blot, ELISA

Synonyms

dsDNA, ssDNA, circular DNA, double stranded DNA, double-stranded DNA

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman placenta tissue, human ovary tumor tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells
Positive Dot Blot detected inN/A

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:800-1:3200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.2 ug per 10^6 cells in a 100 µl suspension
DOT BLOTDOT BLOT : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85854-1-RR targets DNA in IHC, FC (Intra), Dot Blot, ELISA applications and shows reactivity with n/a samples.

Tested Reactivity n/a
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

DNA

相同性解析による交差性が予測される生物種
Full Name DNA
Gene Symbol
Gene ID (NCBI)
RRIDAB_3744424
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DNA antibodies are a type of antibody that can specifically bind to DNA and are mainly used to detect and study DNA-related biological processes. This antibody can recognize single-stranded DNA and double-stranded DNA (including linear DNA and circular DNA).

Protocols

Product Specific Protocols
IHC protocol for DNA antibody 85854-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IHC, FC, ELISA

Toxicology

PFHxS exposure causes osteoarthritis through the innate immune signaling pathway mediated by lipid peroxidation.

Authors - Xicong Chen
View Article
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