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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using DNA Recombinant monoclonal antibody (85854-1-RR)

    IHC staining of human tonsillitis using 85854-1-RR (same clone as 85854-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 85854-1-RR (Anti-DNA antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85854-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using DNA Recombinant monoclonal antibody (85854-1-RR)

    IHC staining of human placenta using 85854-1-RR (same clone as 85854-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85854-1-RR (Anti-DNA antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85854-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using DNA Recombinant monoclonal antibody (85854-1-RR)

    IHC staining of human placenta using 85854-1-RR (same clone as 85854-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85854-1-RR (Anti-DNA antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85854-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using DNA Recombinant monoclonal antibody (85854-1-RR)

    IHC staining of human ovary tumor using 85854-1-RR (same clone as 85854-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 85854-1-RR (Anti-DNA antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85854-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using DNA Recombinant monoclonal antibody (85854-1-RR)

    FC experiment of HeLa using 85854-1-RR (same clone as 85854-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.2 ug N/a Anti-DNA Recombinant Antibody (85854-1-RR, Clone:250864A1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.2 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 85854-1-PBS in a different storage buffer formulation.

  • Dot Blot experiment using DNA Recombinant monoclonal antibody (85854-1-RR)

    Dot Blot experiment using 85854-1-RR (same clone as 85854-1-PBS)

    Dot blot analysis was used to confirm the specificity of 85854-1-RR anti-DNA antibody. samples were spotted onto NC and probed with antibody at 5 µg/ml.The amount of sample (μg) spotted is indicated next to each row. This data was developed using the same antibody clone with 85854-1-PBS in a different storage buffer formulation.

DNA Recombinant monoclonal antibody, PBS Only

DNA Uni-rAb® Recombinant Antibody for IHC, FC (Intra), Dot Blot, Indirect ELISA
Cat No. 85854-1-PBS
Clone No.250864A1

Host / Isotype

Rabbit / IgG

Reactivity

n/a

Applications

IHC, FC (Intra), Dot Blot, Indirect ELISA

Synonyms

circular DNA, double stranded DNA, double-stranded DNA, linear DNA, single stranded DNA

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85854-1-PBS targets DNA in IHC, FC (Intra), Dot Blot, Indirect ELISA applications and shows reactivity with n/a samples.

Tested Reactivity n/a
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

unknow

相同性解析による交差性が予測される生物種
Full Name DNA
Gene Symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DNA antibodies are a type of antibody that can specifically bind to DNA and are mainly used to detect and study DNA-related biological processes. This antibody can recognize single-stranded DNA and double-stranded DNA (including linear DNA and circular DNA).

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