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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated Alpha-2-Macroglobulin Monoclonal antibody

Alpha-2-Macroglobulin Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4B11F7

Cat no : CL594-66126

Synonyms

A2M, Alpha 2 M, alpha 2 macroglobulin, CPAMD5, DKFZp779B086, FWP007, S863 7

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL594-66126

    IF Staining of HepG2 using CL594-66126

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66126 (Alpha-2-macroglobulin antibody) at dilution of 1:100.

Tested Applications

Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy A2M Ready-To-Use IHC Kit. A2M primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66126-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL594-66126 is Alpha-2-Macroglobulin Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Alpha-2-Macroglobulin fusion protein Ag19245 相同性解析による交差性が予測される生物種
Full Name alpha-2-macroglobulin
Calculated molecular weight 1474 aa, 163 kDa
GenBank accession numberBC040071
Gene symbol A2M
Gene ID (NCBI) 2
RRIDAB_2883520
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

alpha-2-macroglobulin, also known as α2-macroglobulin (α2M and A2M), is a protein abundant in the plasma of vertebrates and several invertebrates. A2M is an evolutionarily conserved arm of the innate immune system. It also mediates the proliferation of T cells and macrophages. A2M acts as a nonspecific protease inhibitor involved in the host defense mechanism that inactivates both endogenous and exogenous proteases, including trypsin, thrombin and collagenase. Even though A2M is produced predominantly by the liver, it may also be expressed in the reproductive tract, heart, and brain, and may have important roles in many physiological processes and medical illnesses including Alzheimer's disease.

Protocols

Product Specific Protocols
IF protocol for CL594 Alpha-2-Macroglobulin antibody CL594-66126Download protocol
Standard Protocols
Click here to view our Standard Protocols