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Species Reactivity

Species Reactivity

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Conjugate

  • Featured Product
  • KD/KO Validated

Alix Monoclonal antibody, PBS Only

Alix Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H9D9

Cat no : 67715-1-PBS

Synonyms

AIP1, ALG 2 interacting protein 1, Alix, ALIX; AIP1, DRIP4, HP95, KIAA1375, PDCD6 interacting protein, PDCD6IP

Validation Data Gallery

Applications:
  • WB analysis using 67715-1-Ig (same clone as 67715-1-PBS)

    WB analysis using 67715-1-Ig (same clone as 67715-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67715-1-Ig (Alix antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67715-1-PBS in a different storage buffer formulation.

  • WB analysis using 67715-1-Ig (same clone as 67715-1-PBS)

    WB analysis using 67715-1-Ig (same clone as 67715-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67715-1-Ig (Alix antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67715-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 67715-1-Ig (same clone as 67715-1-PBS)

    WB analysis of HEK-293 using 67715-1-Ig (same clone as 67715-1-PBS)

    WB result of Alix antibody (67715-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Alix transfected HEK-293 cells. This data was developed using the same antibody clone with 67715-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67715-1-Ig (same clone as 67715-1-PBS)

    IF Staining of HeLa using 67715-1-Ig (same clone as 67715-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Alix antibody (67715-1-Ig, Clone: 1H9D9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67715-1-PBS in a different storage buffer formulation.

Tested Applications

Planning an IHC experiment? We recommend our IHCeasy ALIX Ready-To-Use IHC Kit. ALIX primary antibody included.

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

67715-1-PBS targets Alix in WB, IF, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Alix fusion protein Ag30437 相同性解析による交差性が予測される生物種
Full Name programmed cell death 6 interacting protein
Calculated molecular weight 868 aa, 96 kDa
Observed molecular weight100 kDa
GenBank accession numberBC020066
Gene symbol PDCD6IP
Gene ID (NCBI) 10015
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ALG-2-interacting protein 1 (ALIX), also known as AIP1 or Hp95, is encoded by PDCD6IP gene and is involved in cell death through mechanisms involving its binding partner ALG-2 (apoptosis-linked gene-2). ALG-2 is a 22-kDa protein containing five serially repetitive EF-hand structures and is defined as a regulator of calcium-induced apoptosis following endoplasmic reticulum (ER) stress. ALIX interacts with ALG-2 through its C-terminal proline-rich region and participates in formation of multivesicular bodies. Recent finding suggest that ALIX is a critical component of caspase 9 activation and apoptosis triggered by calcium.