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Alix Monoclonal antibody

Alix Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67715-1-Ig
Clone No.1H9D9

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

PDCD6IP, 1H9D9, AIP1, ALG-2-interacting protein 1, ALG-2-interacting protein X

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -


ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。


国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHSC-T6 cells, A431 cells, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 13 publications below

Product Information

67715-1-Ig targets Alix in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Alix fusion protein Ag30437 相同性解析による交差性が予測される生物種
Full Name programmed cell death 6 interacting protein
Calculated molecular weight 868 aa, 96 kDa
Observed molecular weight100 kDa
GenBank accession numberBC020066
Gene Symbol Alix
Gene ID (NCBI) 10015
RRIDAB_2882905
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8WUM4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ALG-2-interacting protein 1 (ALIX), also known as AIP1 or Hp95, is encoded by PDCD6IP gene and is involved in cell death through mechanisms involving its binding partner ALG-2 (apoptosis-linked gene-2). ALG-2 is a 22-kDa protein containing five serially repetitive EF-hand structures and is defined as a regulator of calcium-induced apoptosis following endoplasmic reticulum (ER) stress. ALIX interacts with ALG-2 through its C-terminal proline-rich region and participates in formation of multivesicular bodies. Recent finding suggest that ALIX is a critical component of caspase 9 activation and apoptosis triggered by calcium.

Protocols

Product Specific Protocols
WB protocol for Alix antibody 67715-1-IgDownload protocol
IHC protocol for Alix antibody 67715-1-IgDownload protocol
IF protocol for Alix antibody 67715-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Stem Cell Res Ther

Tremella polysaccharide microneedles loaded with magnetic dental pulp stem cell intracellular vesicles used for androgenic alopecia

Authors - Yonghao Liu
humanWB

Cell Mol Biol Lett

Downregulation of exosomal miR-7-5p promotes breast cancer migration and invasion by targeting RYK and participating in the atypical WNT signalling pathway

Authors - Zhaoyi Liang
mouse,humanWB

J Thromb Haemost

Cancer-associated fibroblasts promote venous thrombosis through podoplanin/CLEC-2 interaction in podoplanin-negative lung cancer mouse model

Authors - Toshiaki Shirai
humanWB

Psychiatry Clin Neurosci

Human in vivo evidence of reduced astrocyte activation and neuroinflammation in patients with treatment-resistant depression following electroconvulsive therapy

Authors - Shu-Xian Xu
humanWB

J Microsc

A method for extraction of exosomes from breast tumour cells and characterisation by transmission electron microscopy

Authors - Zhiang Liu
humanWB

ACS Biomater Sci Eng

Enhancing Skin Injury Repair: Combined Application of PF-127 Hydrogel and hADSC-Exos Containing miR-148a-3p

Authors - Qiqi Zhang
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