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Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using Acetyl-Histone H4 (Lys12) Recombinant antibody (83095-1-RR)

    WB analysis of NIH/3T3 using 83095-1-RR

    Untreated and trichostatin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83095-1-RR (Acetyl-Histone H4 (Lys12) antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Beta Actin Monoclonal antibody (66009-1-Ig) as loading control.

  • Western Blot (WB) analysis of HSC-T6 cells using Acetyl-Histone H4 (Lys12) Recombinant antibody (83095-1-RR)

    WB analysis of HSC-T6 using 83095-1-RR

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 83095-1-RR (Acetyl-Histone H4 (Lys12) antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using Acetyl-Histone H4 (Lys12) Recombinant antibody (83095-1-RR)

    WB analysis of NIH/3T3 using 83095-1-RR

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83095-1-RR (Acetyl-Histone H4 (Lys12) antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using Acetyl-Histone H4 (Lys12) Recombinant antibody (83095-1-RR)

    WB analysis of HEK-293 using 83095-1-RR

    WB result of Acetyl-Histone H4 (Lys12) antibody (83095-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Acetyl-Histone H4 (Lys12) transfected HEK-293 cells.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using Acetyl-Histone H4 (Lys12) Recombinant antibody (83095-1-RR)

    IHC staining of mouse small intestine using 83095-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 83095-1-RR (Acetyl-Histone H4 (Lys12) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using Acetyl-Histone H4 (Lys12) Recombinant antibody (83095-1-RR)

    IHC staining of mouse small intestine using 83095-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 83095-1-RR (Acetyl-Histone H4 (Lys12) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using Acetyl-Histone H4 (Lys12) Recombinant antibody (83095-1-RR)

    IF Staining of Caco-2 using 83095-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using Acetyl-Histone H4 (Lys12) antibody (83095-1-RR, Clone: 1D18 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using Acetyl-Histone H4 (Lys12) Recombinant antibody (83095-1-RR)

    IF Staining of Caco-2 using 83095-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using Acetyl-Histone H4 (Lys12) antibody (83095-1-RR, Clone: 1D18 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Dot Blot experiment of peptide using Acetyl-Histone H4 (Lys12) Recombinant antibody (83095-1-RR)

    Dot Blot experiment of peptide using 83095-1-RR

    Dot blot analysis was used to confirm the specificity of Histone H4AK12ac antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. Column 1: H4AK12ac. Column 2: Unmodified H4AK12. Column 3: H4AK5ac. Column 4: Unmodified H4AK5. Column 5: H4AK8Ac. Column 6: Unmodified H4AK8. Column 7: H4AK16ac. Column 8:Unmodified H4AK16. Column 9: H4AK91ac. Column 10: Unmodified H4K91 Column 11: Blank(PBS).

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  • Featured Product
  • KD/KO Validated

Acetyl-Histone H4 (Lys12) Recombinant antibody

Acetyl-Histone H4 (Lys12) Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Dot Blot, ELISA
Cat No. 83095-1-RR
Clone No.1D18

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Dot Blot, ELISA

Synonyms

Acetyl Histone H4, Ac Histone H4-Lys12, Ac Histone H4 Lys12, Ac Histone H4 (Lys12), Ac Histone H4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inTrichostatin A treated NIH/3T3 cells, HEK-293 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inmouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inCaco-2 cells
Positive Dot Blot detected inpeptide

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
DOT BLOTDOT BLOT : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83095-1-RR targets Acetyl-Histone H4 (Lys12) in WB, IHC, IF/ICC, Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone cluster 1, H4a
Observed molecular weight12 kDa
GenBank accession numberBC069654
Gene Symbol HIST1H4A
Gene ID (NCBI) 8359
RRIDAB_3670809
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP62805
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Histone H4 is a 103 amino acid protein, which belongs to the histone H4 family. Histone H4 localizes in the nucleus and is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Acetylation of histone H4 is necessary for chromatin decompaction during DNA replication.

Protocols

Product Specific Protocols
WB protocol for Acetyl-Histone H4 (Lys12) antibody 83095-1-RRDownload protocol
IHC protocol for Acetyl-Histone H4 (Lys12) antibody 83095-1-RRDownload protocol
IF protocol for Acetyl-Histone H4 (Lys12) antibody 83095-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols