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Validation Data Gallery

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  • Western Blot (WB) analysis of HSC-T6 cells using Acetyl-Histone H4 (Lys12) Recombinant antibody (83095-1-RR)

    WB analysis of HSC-T6 using 83095-1-RR (same clone as 83095-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 83095-1-RR (Acetyl-Histone H4 (Lys12) antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83095-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using Acetyl-Histone H4 (Lys12) Recombinant antibody (83095-1-RR)

    WB analysis of NIH/3T3 using 83095-1-RR (same clone as 83095-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83095-1-RR (Acetyl-Histone H4 (Lys12) antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83095-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using Acetyl-Histone H4 (Lys12) Recombinant antibody (83095-1-RR)

    WB analysis of NIH/3T3 using 83095-1-RR (same clone as 83095-1-PBS)

    Untreated and trichostatin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83095-1-RR (Acetyl-Histone H4 (Lys12) antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Beta Actin Monoclonal antibody (66009-1-Ig) as loading control. This data was developed using the same antibody clone with 83095-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using Acetyl-Histone H4 (Lys12) Recombinant antibody (83095-1-RR)

    WB analysis of HEK-293 using 83095-1-RR (same clone as 83095-1-PBS)

    WB result of Acetyl-Histone H4 (Lys12) antibody (83095-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Acetyl-Histone H4 (Lys12) transfected HEK-293 cells. This data was developed using the same antibody clone with 83095-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using Acetyl-Histone H4 (Lys12) Recombinant antibody (83095-1-RR)

    IHC staining of mouse small intestine using 83095-1-RR (same clone as 83095-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 83095-1-RR (Acetyl-Histone H4 (Lys12) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83095-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using Acetyl-Histone H4 (Lys12) Recombinant antibody (83095-1-RR)

    IHC staining of mouse small intestine using 83095-1-RR (same clone as 83095-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 83095-1-RR (Acetyl-Histone H4 (Lys12) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83095-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using Acetyl-Histone H4 (Lys12) Recombinant antibody (83095-1-RR)

    IF Staining of Caco-2 using 83095-1-RR (same clone as 83095-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using Acetyl-Histone H4 (Lys12) antibody (83095-1-RR, Clone: 1D18 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 83095-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using Acetyl-Histone H4 (Lys12) Recombinant antibody (83095-1-RR)

    IF Staining of Caco-2 using 83095-1-RR (same clone as 83095-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using Acetyl-Histone H4 (Lys12) antibody (83095-1-RR, Clone: 1D18 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83095-1-PBS in a different storage buffer formulation.

  • Dot Blot experiment of peptide using Acetyl-Histone H4 (Lys12) Recombinant antibody (83095-1-RR)

    Dot Blot experiment of peptide using 83095-1-RR (same clone as 83095-1-PBS)

    Dot blot analysis was used to confirm the specificity of Histone H4AK12ac antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. Column 1: H4AK12ac. Column 2: Unmodified H4AK12. Column 3: H4AK5ac. Column 4: Unmodified H4AK5. Column 5: H4AK8Ac. Column 6: Unmodified H4AK8. Column 7: H4AK16ac. Column 8:Unmodified H4AK16. Column 9: H4AK91ac. Column 10: Unmodified H4K91 Column 11: Blank(PBS). This data was developed using the same antibody clone with 83095-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

Acetyl-Histone H4 (Lys12) Recombinant antibody, PBS Only

Acetyl-Histone H4 (Lys12) Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Dot Blot, Indirect ELISA
Cat No. 83095-1-PBS
Clone No.1D18

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Dot Blot, Indirect ELISA

Synonyms

Acetyl Histone H4, Ac Histone H4-Lys12, Ac Histone H4 Lys12, Ac Histone H4 (Lys12), Ac Histone H4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83095-1-PBS targets Acetyl-Histone H4 (Lys12) in WB, IHC, IF/ICC, Dot Blot, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone cluster 1, H4a
Observed molecular weight12 kDa
GenBank accession numberBC069654
Gene Symbol HIST1H4A
Gene ID (NCBI) 8359
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP62805
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Histone H4 is a 103 amino acid protein, which belongs to the histone H4 family. Histone H4 localizes in the nucleus and is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Acetylation of histone H4 is necessary for chromatin decompaction during DNA replication.