Validation Data Gallery

Filter: AllWBIHCIF/ICCDot BlotChIP-qPCR

WB analysis of NIH/3T3 using 83095-1-RR (same clone as 83095-1-PBS)
Untreated and trichostatin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83095-1-RR (Acetyl-Histone H4 (Lys12) antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Beta Actin Monoclonal antibody (66009-1-Ig) as loading control. This data was developed using the same antibody clone with 83095-1-PBS in a different storage buffer formulation.
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Untreated and trichostatin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83095-1-RR (Acetyl-Histone H4 (Lys12) antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Beta Actin Monoclonal antibody (66009-1-Ig) as loading control. This data was developed using the same antibody clone with 83095-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 83095-1-RR (same clone as 83095-1-PBS)
WB result of Acetyl-Histone H4 (Lys12) antibody (83095-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Acetyl-Histone H4 (Lys12) transfected HEK-293 cells. This data was developed using the same antibody clone with 83095-1-PBS in a different storage buffer formulation.
WB analysis of HSC-T6 using 83095-1-RR (same clone as 83095-1-PBS)
HSC-T6 cells were subjected to SDS PAGE followed by western blot with 83095-1-RR (Acetyl-Histone H4 (Lys12) antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83095-1-PBS in a different storage buffer formulation.
WB analysis of NIH/3T3 using 83095-1-RR (same clone as 83095-1-PBS)
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83095-1-RR (Acetyl-Histone H4 (Lys12) antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83095-1-PBS in a different storage buffer formulation.
IHC staining of mouse small intestine using 83095-1-RR (same clone as 83095-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 83095-1-RR (Acetyl-Histone H4 (Lys12) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83095-1-PBS in a different storage buffer formulation.
IHC staining of mouse small intestine using 83095-1-RR (same clone as 83095-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 83095-1-RR (Acetyl-Histone H4 (Lys12) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83095-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 83095-1-RR (same clone as 83095-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83095-1-RR (Acetyl-Histone H4 (Lys12) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83095-1-PBS in a different storage buffer formulation.
IHC staining of mouse colon using 83095-1-RR (same clone as 83095-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83095-1-RR (Acetyl-Histone H4 (Lys12) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83095-1-PBS in a different storage buffer formulation.
IF Staining of Caco-2 using 83095-1-RR (same clone as 83095-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using Acetyl-Histone H4 (Lys12) antibody (83095-1-RR, Clone: 1D18 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83095-1-PBS in a different storage buffer formulation.
IF Staining of Caco-2 using 83095-1-RR (same clone as 83095-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using Acetyl-Histone H4 (Lys12) antibody (83095-1-RR, Clone: 1D18 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 83095-1-PBS in a different storage buffer formulation.
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Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using Acetyl-Histone H4 (Lys12) antibody (83095-1-RR, Clone: 1D18 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 83095-1-PBS in a different storage buffer formulation.
IF Staining of NIH/3T3 using 83095-1-RR (same clone as 83095-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed Trichostatin A treated NIH/3T3 cells using Acetyl-Histone H4 (Lys12) antibody (83095-1-RR, Clone: 1D18 ) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83095-1-PBS in a different storage buffer formulation.
Dot Blot experiment of peptide using 83095-1-RR (same clone as 83095-1-PBS)
Dot blot analysis was used to confirm the specificity of Histone H4AK12ac antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. Column 1: H4AK12ac. Column 2: Unmodified H4AK12. Column 3: H4AK5ac. Column 4: Unmodified H4AK5. Column 5: H4AK8Ac. Column 6: Unmodified H4AK8. Column 7: H4AK16ac. Column 8:Unmodified H4AK16. Column 9: H4AK91ac. Column 10: Unmodified H4K91 Column 11: Blank(PBS). This data was developed using the same antibody clone with 83095-1-PBS in a different storage buffer formulation.
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Dot blot analysis was used to confirm the specificity of Histone H4AK12ac antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. Column 1: H4AK12ac. Column 2: Unmodified H4AK12. Column 3: H4AK5ac. Column 4: Unmodified H4AK5. Column 5: H4AK8Ac. Column 6: Unmodified H4AK8. Column 7: H4AK16ac. Column 8:Unmodified H4AK16. Column 9: H4AK91ac. Column 10: Unmodified H4K91 Column 11: Blank(PBS). This data was developed using the same antibody clone with 83095-1-PBS in a different storage buffer formulation.
ChIP experiment of HeLa using 83095-1-RR (same clone as 83095-1-PBS)
Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H4 (Lys12) (83095-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 83095-1-PBS in a different storage buffer formulation.
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Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H4 (Lys12) (83095-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 83095-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

Acetyl-Histone H4 (Lys12) Recombinant monoclonal antibody, PBS Only

Acetyl-Histone H4 (Lys12) Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR

Cat No. 83095-1-PBS

Clone No.1D18

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR

Synonyms

Ac Histone H4, Ac Histone H4 (Lys12), Ac Histone H4 Lys12, Ac Histone H4-Lys12, Acetyl Histone

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83095-1-PBS targets Acetyl-Histone H4 (Lys12) in WB, IHC, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	histone cluster 1, H4a
Observed molecular weight	12 kDa
GenBank accession number	BC069654
Gene Symbol	HIST1H4A
Gene ID (NCBI)	8359
RRID	AB_3670809
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P62805
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

Histone H4 is a 103 amino acid protein, which belongs to the histone H4 family. Histone H4 localizes in the nucleus and is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Acetylation of histone H4 is necessary for chromatin decompaction during DNA replication.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of NIH/3T3 using 83095-1-RR (same clone as 83095-1-PBS)

WB analysis of HEK-293 using 83095-1-RR (same clone as 83095-1-PBS)

WB analysis of HSC-T6 using 83095-1-RR (same clone as 83095-1-PBS)

WB analysis of NIH/3T3 using 83095-1-RR (same clone as 83095-1-PBS)

IHC staining of mouse small intestine using 83095-1-RR (same clone as 83095-1-PBS)

IHC staining of mouse small intestine using 83095-1-RR (same clone as 83095-1-PBS)

IHC staining of human placenta using 83095-1-RR (same clone as 83095-1-PBS)

IHC staining of mouse colon using 83095-1-RR (same clone as 83095-1-PBS)

IF Staining of Caco-2 using 83095-1-RR (same clone as 83095-1-PBS)

IF Staining of Caco-2 using 83095-1-RR (same clone as 83095-1-PBS)

IF Staining of NIH/3T3 using 83095-1-RR (same clone as 83095-1-PBS)

Dot Blot experiment of peptide using 83095-1-RR (same clone as 83095-1-PBS)

ChIP experiment of HeLa using 83095-1-RR (same clone as 83095-1-PBS)