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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Acetyl-Histone H3 (Lys36) Recombinant monoclonal a (82828-14-RR)

    WB analysis using 82828-14-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82828-14-RR (Acetyl-Histone H3 (Lys36) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Dot Blot experiment using Acetyl-Histone H3 (Lys36) Recombinant monoclonal a (82828-14-RR)

    Dot Blot experiment using 82828-14-RR

    Dot blot analysis was used to confirm the specificity of 82828-14-RR Acetyl-Histone H3(Lys36) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

  • ChIP experiment of HeLa cells using Acetyl-Histone H3 (Lys36) Recombinant antibody (82828-14-RR)

    ChIP experiment of HeLa using 82828-14-RR

    Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of HIST1H3A (82828-14-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

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Acetyl-Histone H3 (Lys36) Recombinant monoclonal antibody

Acetyl-Histone H3 (Lys36) Uni-rAb® Recombinant Antibody for WB, Dot Blot, ELISA, ChIP-qPCR
Cat No. 82828-14-RR
Clone No.250370A9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Dot Blot, ELISA, ChIP-qPCR

Synonyms

Ac Histone H3, Ac Histone H3 (Lys36), Ac Histone H3 Lys36, Ac Histone H3-Lys36, Acetyl Histone

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inTrichostatin A treated NIH/3T3 cells
Positive Dot Blot detected inN/A N/A
Positive ChIP-qPCR detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
DOT BLOTDOT BLOT : 1:10-1:100
CHIP-QPCRCHIP-QPCR : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82828-14-RR targets Acetyl-Histone H3 (Lys36) in WB, Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone cluster 1, H3a
Observed molecular weight15 kDa
GenBank accession numberBC066245
Gene Symbol HIST1H3A
Gene ID (NCBI) 8350
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP68431
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

H3K36ac peaks at the 5' end of genes,manily on the two nucleosomeus immediately distal to the transcription start site, independently of gene length. H3K36ac and H3K36mac showed negative creastalk, which is mediated by GCN3 and the histone methyl transferase SDX8. Although H3K36ac is associated with gene activity, there is no linear relationship between H3K36ac and transcript levels, suggesting that H3K36ac is a binary indicator of transcription.

Protocols

Product Specific Protocols
WB protocol for Acetyl-Histone H3 (Lys36) antibody 82828-14-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols