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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Acetyl-Histone H3 (Lys36) Recombinant monoclonal a (82828-14-RR)

    WB analysis using 82828-14-RR (same clone as 82828-14-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82828-14-RR (Acetyl-Histone H3 (Lys36) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82828-14-PBS in a different storage buffer formulation.

  • Dot Blot experiment using Acetyl-Histone H3 (Lys36) Recombinant monoclonal a (82828-14-RR)

    Dot Blot experiment using 82828-14-RR (same clone as 82828-14-PBS)

    Dot blot analysis was used to confirm the specificity of 82828-14-RR Acetyl-Histone H3(Lys36) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 82828-14-PBS in a different storage buffer formulation.

  • ChIP experiment of HeLa cells using Acetyl-Histone H3 (Lys36) Recombinant monoclonal a (82828-14-RR)

    ChIP experiment of HeLa using 82828-14-RR (same clone as 82828-14-PBS)

    Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of HIST1H3A (82828-14-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 82828-14-PBS in a different storage buffer formulation.

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Acetyl-Histone H3 (Lys36) Recombinant monoclonal antibody, PBS Only

Acetyl-Histone H3 (Lys36) Uni-rAb® Recombinant Antibody for WB, Dot Blot, Indirect ELISA, ChIP-qPCR
Cat No. 82828-14-PBS
Clone No.250370A9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Dot Blot, Indirect ELISA, ChIP-qPCR

Synonyms

Ac Histone H3, Ac Histone H3 (Lys36), Ac Histone H3 Lys36, Ac Histone H3-Lys36, Acetyl Histone

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82828-14-PBS targets Acetyl-Histone H3 (Lys36) in WB, Dot Blot, Indirect ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone cluster 1, H3a
Observed molecular weight15 kDa
GenBank accession numberBC066245
Gene Symbol HIST1H3A
Gene ID (NCBI) 8350
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP68431
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

H3K36ac peaks at the 5' end of genes,manily on the two nucleosomeus immediately distal to the transcription start site, independently of gene length. H3K36ac and H3K36mac showed negative creastalk, which is mediated by GCN3 and the histone methyl transferase SDX8. Although H3K36ac is associated with gene activity, there is no linear relationship between H3K36ac and transcript levels, suggesting that H3K36ac is a binary indicator of transcription.