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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATPBD4 Monoclonal antibody (68397-1-Ig)

    WB analysis using 68397-1-Ig (same clone as 68397-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68397-1-Ig (ATPBD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68397-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using ATPBD4 Monoclonal antibody (68397-1-Ig)

    WB analysis of K-562 using 68397-1-Ig (same clone as 68397-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 68397-1-Ig (ATPBD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68397-1-PBS in a different storage buffer formulation.

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ATPBD4 Monoclonal antibody, PBS Only

ATPBD4 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68397-1-PBS
Clone No.2B12E1

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Indirect ELISA

Synonyms

ATP binding domain 4, ATPBD4, DPH6

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68397-1-PBS targets ATPBD4 in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATPBD4 fusion protein Ag33218 相同性解析による交差性が予測される生物種
Full Name ATP binding domain 4
Calculated molecular weight 267 aa, 30 kDa
Observed molecular weight 28-30 kDa
GenBank accession numberBC008485
Gene Symbol ATPBD4
Gene ID (NCBI) 89978
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ7L8W6
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Diphthamide is a highly modified histidine in the transcription factor EEF2 that is conserved from yeast to human. ATPBD4, also known as DPH6, belongs to the Diphthine--ammonia ligase family. DPH6 amidase may catalyze the last step of diphthamide biosynthesis using ammonium and ATP to play a role in catalyzing the final step of diphthamide biosynthesis, amidation of diphthine to diphthamide (PubMed:23169644).