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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATPBD4 Monoclonal antibody (68397-1-Ig)

    WB analysis using 68397-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68397-1-Ig (ATPBD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of K-562 cells using ATPBD4 Monoclonal antibody (68397-1-Ig)

    WB analysis of K-562 using 68397-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 68397-1-Ig (ATPBD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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ATPBD4 Monoclonal antibody

ATPBD4 Monoclonal Antibody for WB, ELISA
Cat No. 68397-1-Ig
Clone No.2B12E1

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Synonyms

ATP binding domain 4, ATPBD4, DPH6

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, K-562 cells, LNCaP cells, HeLa cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68397-1-Ig targets ATPBD4 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATPBD4 fusion protein Ag33218 相同性解析による交差性が予測される生物種
Full Name ATP binding domain 4
Calculated molecular weight 267 aa, 30 kDa
Observed molecular weight 28-30 kDa
GenBank accession numberBC008485
Gene Symbol ATPBD4
Gene ID (NCBI) 89978
RRIDAB_3085114
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ7L8W6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Diphthamide is a highly modified histidine in the transcription factor EEF2 that is conserved from yeast to human. ATPBD4, also known as DPH6, belongs to the Diphthine--ammonia ligase family. DPH6 amidase may catalyze the last step of diphthamide biosynthesis using ammonium and ATP to play a role in catalyzing the final step of diphthamide biosynthesis, amidation of diphthine to diphthamide (PubMed:23169644).

Protocols

Product Specific Protocols
WB protocol for ATPBD4 antibody 68397-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols