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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse spleen tissue using ATP9A Polyclonal antibody (19504-1-AP)

    WB analysis of mouse spleen using 19504-1-AP (same clone as 19504-1-PBS)

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 19504-1-AP (ATP9A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19504-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using ATP9A Polyclonal antibody (19504-1-AP)

    IHC staining of human testis using 19504-1-AP (same clone as 19504-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19504-1-AP (ATP9A Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 19504-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using ATP9A Polyclonal antibody (19504-1-AP)

    IHC staining of human testis using 19504-1-AP (same clone as 19504-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19504-1-AP (ATP9A Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 19504-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using ATP9A Polyclonal antibody (19504-1-AP)

    IHC staining of rat brain using 19504-1-AP (same clone as 19504-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 19504-1-AP (ATP9A Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 19504-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using ATP9A Polyclonal antibody (19504-1-AP)

    IHC staining of rat brain using 19504-1-AP (same clone as 19504-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 19504-1-AP (ATP9A Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 19504-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ATP9A Polyclonal antibody (19504-1-AP)

    IF Staining of HeLa using 19504-1-AP (same clone as 19504-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 19504-1-AP (ATP9A antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19504-1-PBS in a different storage buffer formulation.

ATP9A Polyclonal antibody, PBS Only

ATP9A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 19504-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19504-1-PBS targets ATP9A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name ATPase, class II, type 9A
Calculated molecular weight 119 kDa
Observed molecular weight 105 kDa
GenBank accession numberNM_006045
Gene Symbol ATP9A
Gene ID (NCBI) 10079
RRIDAB_10640806
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO75110
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ATP9A, also named as ATPIIA and KIAA0611, belongs to the cation transport ATPase (P-type) family and Type IV subfamily. ATP9A catalyzes the reaction: ATP + H2O + phospholipid(In) = ADP + phosphate + phospholipid(Out).

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