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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse spleen tissue using ATP9A Polyclonal antibody (19504-1-AP)

    WB analysis of mouse spleen using 19504-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 19504-1-AP (ATP9A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using ATP9A Polyclonal antibody (19504-1-AP)

    IHC staining of human testis using 19504-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19504-1-AP (ATP9A Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ATP9A Polyclonal antibody (19504-1-AP)

    IHC staining of human testis using 19504-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19504-1-AP (ATP9A Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of rat brain tissue using ATP9A Polyclonal antibody (19504-1-AP)

    IHC staining of rat brain using 19504-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 19504-1-AP (ATP9A Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of rat brain tissue using ATP9A Polyclonal antibody (19504-1-AP)

    IHC staining of rat brain using 19504-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 19504-1-AP (ATP9A Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ATP9A Polyclonal antibody (19504-1-AP)

    IF Staining of HeLa using 19504-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 19504-1-AP (ATP9A antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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ATP9A Polyclonal antibody

ATP9A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 19504-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Probable phospholipid-transporting ATPase IIA, KIAA0611, EC:7.6.2.1, ATPIIA

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse spleen tissue
Positive IHC detected inhuman testis tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19504-1-AP targets ATP9A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name ATPase, class II, type 9A
Calculated molecular weight 119 kDa
Observed molecular weight 105 kDa
GenBank accession numberNM_006045
Gene Symbol ATP9A
Gene ID (NCBI) 10079
RRIDAB_10640806
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75110
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATP9A, also named as ATPIIA and KIAA0611, belongs to the cation transport ATPase (P-type) family and Type IV subfamily. ATP9A catalyzes the reaction: ATP + H2O + phospholipid(In) = ADP + phosphate + phospholipid(Out).

Protocols

Product Specific Protocols
WB protocol for ATP9A antibody 19504-1-APDownload protocol
IHC protocol for ATP9A antibody 19504-1-APDownload protocol
IF protocol for ATP9A antibody 19504-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols