Validation Data Gallery
Tested Applications
| Positive WB detected in | 37°C incubated HEK-293 cells, 37°C incubated mouse liver tissue |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86762-3-RR targets ATP13A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag9174 Product name: Recombinant human ATP13A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 615-758 aa of BC009302 Sequence: QKEFVITSLKELGYVTLMCGDGTNDVGALKHADVGVALLANAPERVVERRRRPRDSPTLSNSGIRATSRTAKQRSGLPPSEEQPTSQRDRLSQVLRDLEDESTPIVKLGDASIAAPFTSKLSSIQCICHVIKQGRCTLVTTLQM 相同性解析による交差性が予測される生物種 |
| Full Name | ATPase type 13A1 |
| Calculated molecular weight | 976 aa, 109 kDa |
| Observed molecular weight | 120-130 kDa |
| GenBank accession number | BC009302 |
| Gene Symbol | ATP13A1 |
| Gene ID (NCBI) | 57130 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9HD20 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ATP13A1 is a P-type ATPase, mainly involved in the transport of cations within cells. It plays an important role in maintaining intracellular ion homeostasis and membrane potential. ATP13A1 acts as a dislocase that mediates the ATP-dependent extraction of mislocalized mitochondrial transmembrane proteins from the endoplasmic reticulum membrane (PubMed:32973005).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ATP13A1 antibody 86762-3-RR | Download protocol |
| IHC protocol for ATP13A1 antibody 86762-3-RR | Download protocol |
| WB protocol for ATP13A1 antibody 86762-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |