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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using ATP13A1 Recombinant monoclonal antibody (86762-3-RR)

    WB analysis of HEK-293 using 86762-3-RR (same clone as 86762-3-PBS)

    37°C incubated HEK-293 cells were subjected to SDS PAGE followed by western blot with 86762-3-RR (ATP13A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86762-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using ATP13A1 Recombinant monoclonal antibody (86762-3-RR)

    WB analysis of mouse liver using 86762-3-RR (same clone as 86762-3-PBS)

    37°C incubated mouse liver tissue were subjected to SDS PAGE followed by western blot with 86762-3-RR (ATP13A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86762-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ATP13A1 Recombinant monoclonal antibody (86762-3-RR)

    IHC staining of mouse liver using 86762-3-RR (same clone as 86762-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 86762-3-RR (ATP13A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86762-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ATP13A1 Recombinant monoclonal antibody (86762-3-RR)

    IHC staining of mouse liver using 86762-3-RR (same clone as 86762-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 86762-3-RR (ATP13A1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86762-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ATP13A1 Recombinant monoclonal antibody (86762-3-RR)

    IF Staining of HeLa using 86762-3-RR (same clone as 86762-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATP13A1 antibody (86762-3-RR, Clone: 251765A3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86762-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86762-3-RR

    Affinity and Kinetic Characterization of 86762-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86762-3-RR against Human ATP13A1 were performed. The affinity constant is 0.629 nM.

ATP13A1 Recombinant monoclonal antibody, PBS Only

ATP13A1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 86762-3-PBS
Clone No.251765A3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ATP13A, CGI-152, EC:7.4.2.-, Endoplasmic reticulum P5A-ATPase, Endoplasmic reticulum transmembrane helix translocase

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86762-3-PBS targets ATP13A1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag9174

Product name: Recombinant human ATP13A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 615-758 aa of BC009302

Sequence: QKEFVITSLKELGYVTLMCGDGTNDVGALKHADVGVALLANAPERVVERRRRPRDSPTLSNSGIRATSRTAKQRSGLPPSEEQPTSQRDRLSQVLRDLEDESTPIVKLGDASIAAPFTSKLSSIQCICHVIKQGRCTLVTTLQM

相同性解析による交差性が予測される生物種
Full Name ATPase type 13A1
Calculated molecular weight 976 aa, 109 kDa
Observed molecular weight120-130 kDa
GenBank accession numberBC009302
Gene Symbol ATP13A1
Gene ID (NCBI) 57130
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9HD20
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ATP13A1 is a P-type ATPase, mainly involved in the transport of cations within cells. It plays an important role in maintaining intracellular ion homeostasis and membrane potential. ATP13A1 acts as a dislocase that mediates the ATP-dependent extraction of mislocalized mitochondrial transmembrane proteins from the endoplasmic reticulum membrane (PubMed:32973005).

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