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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATAT1 Polyclonal antibody (28828-1-AP)

    WB analysis using 28828-1-AP (same clone as 28828-1-PBS)

    U-251 cells were subjected to SDS PAGE followed by western blot with 28828-1-AP (ATAT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28828-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ATAT1 Polyclonal antibody (28828-1-AP)

    WB analysis using 28828-1-AP (same clone as 28828-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28828-1-AP (ATAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28828-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using ATAT1 Polyclonal antibody (28828-1-AP)

    IHC staining of rat testis using 28828-1-AP (same clone as 28828-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28828-1-AP (ATAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28828-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using ATAT1 Polyclonal antibody (28828-1-AP)

    IHC staining of rat testis using 28828-1-AP (same clone as 28828-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28828-1-AP (ATAT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28828-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using ATAT1 Polyclonal antibody (28828-1-AP)

    IHC staining of mouse testis using 28828-1-AP (same clone as 28828-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28828-1-AP (ATAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28828-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using ATAT1 Polyclonal antibody (28828-1-AP)

    IHC staining of mouse testis using 28828-1-AP (same clone as 28828-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28828-1-AP (C6orf134 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28828-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using ATAT1 Polyclonal antibody (28828-1-AP)

    IF Staining of A549 using 28828-1-AP (same clone as 28828-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ATAT1 antibody (28828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28828-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using ATAT1 Polyclonal antibody (28828-1-AP)

    IF Staining of A549 using 28828-1-AP (same clone as 28828-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ATAT1 antibody (28828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28828-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using ATAT1 Polyclonal antibody (28828-1-AP)

    IF Staining of A549 using 28828-1-AP (same clone as 28828-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ATAT1 antibody (28828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 28828-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using ATAT1 Polyclonal antibody (28828-1-AP)

    IF Staining of A549 using 28828-1-AP (same clone as 28828-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ATAT1 antibody (28828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 28828-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using ATAT1 Polyclonal antibody (28828-1-AP)

    FC experiment of A431 using 28828-1-AP (same clone as 28828-1-PBS)

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human ATAT1 (28828-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 28828-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ATAT1 Polyclonal antibody, PBS Only

ATAT1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 28828-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

C6orf134, Alpha-TAT, alpha-TAT1, FLJ13158, MEC17

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28828-1-PBS targets ATAT1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag28757

Product name: Recombinant human C6orf134 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 80-325 aa of NM_001031722

Sequence: MLKDSSARPAGKGAIIGFIKVGYKKLFVLDDREAHNEVEPLCILDFYIHESVQRHGHGRELFQYMLQKERVEPHQLAIDRPSQKLLKFLNKHYNLETTVPQVNNFVIFEGFFAHQHRPPAPSLRATRHSRAAAVDPTPAAPARKLPPKRAEGDIKPYSSSDREFLKVAVEPPWPLNRAPRRATPPAHPPPRSSSLGNSPERGPLRPFVPEQELLRSLRLCPPHPTARLLLAADPGGSPAQRRRTRGT

相同性解析による交差性が予測される生物種
Full Name chromosome 6 open reading frame 134
Calculated molecular weight 47 kDa
Observed molecular weight42-45 kDa
GenBank accession numberNM_001031722
Gene Symbol ATAT1
Gene ID (NCBI) 79969
RRIDAB_2881219
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5SQI0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

C6orf134 is a reported alias name for the human gene ATAT1 coding alpha-tubulin N-acetyltransferase 1, and specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. May affect microtubule stability and regulate microtubule dynamics. May be involved in neuron development. Western blot shows a band between 36 kDa and 45 kDa.

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