Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, U-251 cells, THP-1 cells, U-87 MG cells |
| Positive IHC detected in | rat testis tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
| Positive FC (Intra) detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 7 publications below |
| IF | See 3 publications below |
Product Information
28828-1-AP targets ATAT1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28757 Product name: Recombinant human C6orf134 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 80-325 aa of NM_001031722 Sequence: MLKDSSARPAGKGAIIGFIKVGYKKLFVLDDREAHNEVEPLCILDFYIHESVQRHGHGRELFQYMLQKERVEPHQLAIDRPSQKLLKFLNKHYNLETTVPQVNNFVIFEGFFAHQHRPPAPSLRATRHSRAAAVDPTPAAPARKLPPKRAEGDIKPYSSSDREFLKVAVEPPWPLNRAPRRATPPAHPPPRSSSLGNSPERGPLRPFVPEQELLRSLRLCPPHPTARLLLAADPGGSPAQRRRTRGT 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 6 open reading frame 134 |
| Calculated molecular weight | 47 kDa |
| Observed molecular weight | 42-45 kDa |
| GenBank accession number | NM_001031722 |
| Gene Symbol | ATAT1 |
| Gene ID (NCBI) | 79969 |
| RRID | AB_2881219 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5SQI0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
C6orf134 is a reported alias name for the human gene ATAT1 coding alpha-tubulin N-acetyltransferase 1, and specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. May affect microtubule stability and regulate microtubule dynamics. May be involved in neuron development. Western blot shows a band between 36 kDa and 45 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ATAT1 antibody 28828-1-AP | Download protocol |
| IHC protocol for ATAT1 antibody 28828-1-AP | Download protocol |
| WB protocol for ATAT1 antibody 28828-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
iScience MFN2 coordinates mitochondria motility with α-tubulin acetylation and this regulation is disrupted in CMT2A | ||
Biochem Biophys Res Commun NWD1 influences the extension of neuronal axons by regulating microtubule stability | ||
Cancer Cell Int Suppression of microtubule acetylation mediates the anti-leukemic effect of CDK9 inhibition
| ||
Cell Death Dis TMEM232 is required for the formation of sperm flagellum and male fertility in mice | ||
Clin Transl Med Pathogenic variants of TUBB8 cause oocyte spindle defects by disrupting with EB1/CAKP5 interactions and potential treatment targeting microtubule acetylation through HDAC6 inhibition | ||
Sci China Life Sci FSD1 inhibits glioblastoma diffuse infiltration through restriction of HDAC6-mediated microtubule deacetylation |