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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATAD3A/B Recombinant antibody (86461-2-RR)

    WB analysis using 86461-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86461-2-RR (ATAD3B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEPG2 cells using ATAD3A/B Recombinant antibody (86461-2-RR)

    IF Staining of HEPG2 using 86461-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HEPG2 cells using ATAD3A/B antibody (86461-2-RR, Clone: 251342C9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 86461-2-RR

    Affinity and Kinetic Characterization of 86461-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86461-2-RR against Human ATAD3A/B were performed. The affinity constant is 0.654 nM.

ATAD3A/B Recombinant monoclonal antibody

ATAD3A/B Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86461-2-RR
Clone No.251342C9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

ATAD3B, AAA TOB3, AAATOB3, AAA-TOB3, ATAD

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, U-251 cells, mouse kidney tissue, rat kidney tissue, mouse brain tissue
Positive IF/ICC detected inHEPG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86461-2-RR targets ATAD3A/B in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ATAD3A/B fusion protein Ag9836 相同性解析による交差性が予測される生物種
Full Name ATPase family, AAA domain containing 3B
Calculated molecular weight 72 kDa
Observed molecular weight65-70 kDa
GenBank accession numberBC002542
Gene Symbol ATAD3B
Gene ID (NCBI) 83858
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ5T9A4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATAD3 (ATPase family AAA Domain-containing protein 3) is a mitochondrial membrane bound ATPase whose function has not yet been discovered but its role is essential for embryonic development. The analysis of the cellular properties of ATAD3A and ATAD3B in different human cancer cell lines shows on the contrary that they can present anti-proliferative and chemoresistant properties.

Protocols

Product Specific Protocols
WB protocol for ATAD3A/B antibody 86461-2-RRDownload protocol
IF protocol for ATAD3A/B antibody 86461-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols