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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATAD3A/B Recombinant antibody (86461-2-RR)

    WB analysis using 86461-2-RR (same clone as 86461-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86461-2-RR (ATAD3B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86461-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEPG2 cells using ATAD3A/B Recombinant antibody (86461-2-RR)

    IF Staining of HEPG2 using 86461-2-RR (same clone as 86461-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEPG2 cells using ATAD3A/B antibody (86461-2-RR, Clone: 251342C9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86461-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86461-2-RR

    Affinity and Kinetic Characterization of 86461-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86461-2-RR against Human ATAD3A/B were performed. The affinity constant is 0.654 nM.

ATAD3A/B Recombinant monoclonal antibody, PBS Only

ATAD3A/B Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86461-2-PBS
Clone No.251342C9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

ATAD3B, AAA TOB3, AAATOB3, AAA-TOB3, ATAD

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86461-2-PBS targets ATAD3A/B in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag9836

Product name: Recombinant human ATAD3B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-270 aa of BC002542

Sequence: MSWLFGVNKGPKGEGAGPPPPLPPAQPGAEGGGDRGLGDRPAPKDKWSNFDPTGLERAAKAARELEHSRYAKEALNLAQMQEQTLQLEQQSKLKEYEAAVEQLKSEQIRAQAEERRKTLSEETRQHQARAQYQDKLARQRYEDQLKQQRLLNEENLRKQEESVQKQEAMRRATVEREMELRHKNEMLRVETEARARAKAERENADIIREQIRLKASEHRQTVLESIRTAGTLFGEGFRAFVTDRDKVTATVAGLTLLAVGVYSAKNATAV

相同性解析による交差性が予測される生物種
Full Name ATPase family, AAA domain containing 3B
Calculated molecular weight 72 kDa
Observed molecular weight65-70 kDa
GenBank accession numberBC002542
Gene Symbol ATAD3B
Gene ID (NCBI) 83858
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ5T9A4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ATAD3 (ATPase family AAA Domain-containing protein 3) is a mitochondrial membrane bound ATPase whose function has not yet been discovered but its role is essential for embryonic development. The analysis of the cellular properties of ATAD3A and ATAD3B in different human cancer cell lines shows on the contrary that they can present anti-proliferative and chemoresistant properties.