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Target

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Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated ASNS Polyclonal antibody

ASNS Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-14681

Synonyms

ASNS, asparagine synthetase, TS11

Validation Data Gallery

Applications:
  • IF Staining of SH-SY5Y using CL488-14681

    IF Staining of SH-SY5Y using CL488-14681

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CoraLite® Plus 488 ASNS antibody (CL488-14681) at dilution of 1:200.

Tested Applications

Positive IF detected inSH-SY5Y cells
For other applications, we recommend the unconjugated version of this antibody, 14681-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-14681 targets ASNS in IF applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASNS fusion protein Ag6393 相同性解析による交差性が予測される生物種
Full Name asparagine synthetase
Calculated molecular weight 64 kDa
Observed molecular weight 55-64 kDa
GenBank accession numberBC014621
Gene symbol ASNS
Gene ID (NCBI) 440
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Asparagine synthetase [glutamine-hydrolyzing](ASNS) is also named as TS11. The asparagine synthetase gene, encoding the enzyme that catalyzes the synthesis of asparagine and glutamate using glutamine and aspartate, is a gene for which transcription is highly regulated by the nutritional status of the cell(PMID:15385533). ASNS has some isoforms with the MW of 55-64 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ASNS antibody CL488-14681Download protocol
Standard Protocols
Click here to view our Standard Protocols