Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | HeLa cells, HCT 116 cells, K-562 cells, U2OS cells, mouse testis tissue, rat testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85892-1-RR targets ASNS in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | ASNS fusion protein Ag6393 相同性解析による交差性が予測される生物種 |
| Full Name | asparagine synthetase |
| Calculated molecular weight | 64 kDa |
| Observed molecular weight | 55-64 kDa |
| GenBank accession number | BC014621 |
| Gene Symbol | ASNS |
| Gene ID (NCBI) | 440 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P08243 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Asparagine synthetase [glutamine-hydrolyzing](ASNS) is also named as TS11. The asparagine synthetase gene, encoding the enzyme that catalyzes the synthesis of asparagine and glutamate using glutamine and aspartate, is a gene for which transcription is highly regulated by the nutritional status of the cell(PMID:15385533). ASNS has some isoforms with the MW of 55-64 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ASNS antibody 85892-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |