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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ASNS Recombinant antibody (85892-1-RR)

    WB analysis using 85892-1-RR (same clone as 85892-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85892-1-RR (ASNS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85892-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ASNS Recombinant antibody (85892-1-RR)

    WB analysis using 85892-1-RR (same clone as 85892-1-PBS)

    Cells and lysate were subjected to SDS PAGE followed by western blot with 85892-1-RR (ASNS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85892-1-PBS in a different storage buffer formulation.

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ASNS Recombinant antibody, PBS Only

ASNS Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 85892-1-PBS
Clone No.250177C6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Asparagine synthetase [glutamine-hydrolyzing], EC:6.3.5.4, Glutamine-dependent asparagine synthetase, TS11

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85892-1-PBS targets ASNS in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ASNS fusion protein Ag6393 相同性解析による交差性が予測される生物種
Full Name asparagine synthetase
Calculated molecular weight 64 kDa
Observed molecular weight 55-64 kDa
GenBank accession numberBC014621
Gene Symbol ASNS
Gene ID (NCBI) 440
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08243
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Asparagine synthetase [glutamine-hydrolyzing](ASNS) is also named as TS11. The asparagine synthetase gene, encoding the enzyme that catalyzes the synthesis of asparagine and glutamate using glutamine and aspartate, is a gene for which transcription is highly regulated by the nutritional status of the cell(PMID:15385533). ASNS has some isoforms with the MW of 55-64 kDa.