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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse thymus tissue using ASF1A/B Polyclonal antibody (11011-1-AP)

    WB analysis of mouse thymus using 11011-1-AP (same clone as 11011-1-PBS)

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11011-1-AP (ASF1B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11011-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ASF1A/B Polyclonal antibody (11011-1-AP)

    WB analysis of HeLa using 11011-1-AP (same clone as 11011-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 11011-1-AP (ASF1B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11011-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse thymus tissue using ASF1A/B Polyclonal antibody (11011-1-AP)

    WB analysis of mouse thymus using 11011-1-AP (same clone as 11011-1-PBS)

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11011-1-AP (ASF1B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11011-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse thymus tissue using ASF1A/B Polyclonal antibody (11011-1-AP)

    WB analysis of mouse thymus using 11011-1-AP (same clone as 11011-1-PBS)

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11011-1-AP (ASF1B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11011-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using ASF1A/B Polyclonal antibody (11011-1-AP)

    IHC staining of human lymphoma using 11011-1-AP (same clone as 11011-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11011-1-AP (ASF1A/B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11011-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using ASF1A/B Polyclonal antibody (11011-1-AP)

    IHC staining of human lymphoma using 11011-1-AP (same clone as 11011-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11011-1-AP (ASF1A/B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11011-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ASF1A/B Polyclonal antibody (11011-1-AP)

    IF Staining of HeLa using 11011-1-AP (same clone as 11011-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 11011-1-AP (ASF1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11011-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11011-1-PBS targets ASF1A/B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASF1A/B fusion protein Ag1477 相同性解析による交差性が予測される生物種
Full Name ASF1 anti-silencing function 1 homolog B (S. cerevisiae)
Calculated molecular weight 202 aa, 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC010014
Gene Symbol ASF1B
Gene ID (NCBI) 55723
RRIDAB_2059670
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NVP2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ASF1B is a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly.This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly. This antibody reacts with the ASF1B and ASF1A protein.