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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ASF1A/B Polyclonal antibody

ASF1A/B Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11011-1-AP

Synonyms

ASF1A/B, ASF1B, CCG1 interacting factor A II, CIA II, hAsf1, hAsf1b, hCIA II, Histone chaperone ASF1B

Validation Data Gallery

Applications:
  • WB analysis of mouse thymus using 11011-1-AP

    WB analysis of mouse thymus using 11011-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11011-1-AP (ASF1B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse thymus using 11011-1-AP

    WB analysis of mouse thymus using 11011-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11011-1-AP (ASF1B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse thymus using 11011-1-AP

    WB analysis of mouse thymus using 11011-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11011-1-AP (ASF1B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 11011-1-AP

    WB analysis of HeLa using 11011-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11011-1-AP (ASF1B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 11011-1-AP

    IHC staining of human lymphoma using 11011-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11011-1-AP (ASF1A/B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ASF1B antibody included
  • IHC staining of human lymphoma using 11011-1-AP

    IHC staining of human lymphoma using 11011-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11011-1-AP (ASF1A/B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ASF1B antibody included
  • IF Staining of HeLa using 11011-1-AP

    IF Staining of HeLa using 11011-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 11011-1-AP (ASF1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse thymus tissue, HeLa cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ASF1B Ready-To-Use IHC Kit. ASF1B primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11011-1-AP targets ASF1A/B in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASF1A/B fusion protein Ag1477 相同性解析による交差性が予測される生物種
Full Name ASF1 anti-silencing function 1 homolog B (S. cerevisiae)
Calculated molecular weight 202 aa, 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC010014
Gene symbol ASF1B
Gene ID (NCBI) 55723
RRIDAB_2059670
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ASF1B is a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly.This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly. This antibody reacts with the ASF1B and ASF1A protein.

Protocols

Product Specific Protocols
WB protocol for ASF1A/B antibody 11011-1-APDownload protocol
IHC protocol for ASF1A/B antibody 11011-1-APDownload protocol
IF protocol for ASF1A/B antibody 11011-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols