Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, mouse brain tissue, HepG2 cells, K-562 cells, MCF-7 cells, HEK-293T cells, rat brain tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:150-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86086-1-RR targets ASCC2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | ASCC2 fusion protein Ag2100 相同性解析による交差性が予測される生物種 |
| Full Name | activating signal cointegrator 1 complex subunit 2 |
| Calculated molecular weight | 757 aa, 86 kDa |
| Observed molecular weight | 90-100 kDa |
| GenBank accession number | BC025368 |
| Gene Symbol | ASCC2 |
| Gene ID (NCBI) | 84164 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9H1I8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ASCC2, also known as ASC1p100, is a subunit of the activating signal cointegrator 1 complex. ASCC2 plays a role in DNA damage repair as a component of the ASCC complex, recruiting ASCC3 and ALKBH3 to sites of DNA damage by binding to polyubiquitinated proteins that have 'Lys-63'-linked polyubiquitin chains. Additionally, it is part of the ASC-1 complex that enhances NF-kappa-B, SRF, and AP1 transactivation(PMID: 29144457, 12077347).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ASCC2 antibody 86086-1-RR | Download protocol |
| IHC protocol for ASCC2 antibody 86086-1-RR | Download protocol |
| IF protocol for ASCC2 antibody 86086-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |