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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ASCC2 Recombinant antibody (86086-1-RR)

    WB analysis using 86086-1-RR (same clone as 86086-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86086-1-RR (ASCC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86086-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ASCC2 Recombinant antibody (86086-1-RR)

    WB analysis using 86086-1-RR (same clone as 86086-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86086-1-RR (ASCC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86086-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ASCC2 Recombinant antibody (86086-1-RR)

    IHC staining of human colon cancer using 86086-1-RR (same clone as 86086-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86086-1-RR (ASCC2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86086-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ASCC2 Recombinant antibody (86086-1-RR)

    IF Staining of HepG2 using 86086-1-RR (same clone as 86086-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ASCC2 antibody (86086-1-RR, Clone: 250566D5 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86086-1-PBS in a different storage buffer formulation.

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ASCC2 Recombinant antibody, PBS Only

ASCC2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 86086-1-PBS
Clone No.250566D5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ASC-1 complex subunit p100, ASC1P100, RQT3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86086-1-PBS targets ASCC2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ASCC2 fusion protein Ag2100 相同性解析による交差性が予測される生物種
Full Name activating signal cointegrator 1 complex subunit 2
Calculated molecular weight 757 aa, 86 kDa
Observed molecular weight 90-100 kDa
GenBank accession numberBC025368
Gene Symbol ASCC2
Gene ID (NCBI) 84164
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H1I8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ASCC2, also known as ASC1p100, is a subunit of the activating signal cointegrator 1 complex. ASCC2 plays a role in DNA damage repair as a component of the ASCC complex, recruiting ASCC3 and ALKBH3 to sites of DNA damage by binding to polyubiquitinated proteins that have 'Lys-63'-linked polyubiquitin chains. Additionally, it is part of the ASC-1 complex that enhances NF-kappa-B, SRF, and AP1 transactivation(PMID: 29144457, 12077347).