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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ASAP3 Polyclonal antibody (14099-1-AP)

    WB analysis using 14099-1-AP (same clone as 14099-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14099-1-AP (ASAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14099-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using ASAP3 Polyclonal antibody (14099-1-AP)

    IHC staining of mouse lung using 14099-1-AP (same clone as 14099-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 14099-1-AP (ASAP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14099-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using ASAP3 Polyclonal antibody (14099-1-AP)

    IHC staining of mouse lung using 14099-1-AP (same clone as 14099-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 14099-1-AP (ASAP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14099-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ASAP3 Polyclonal antibody (14099-1-AP)

    IF Staining of HepG2 using 14099-1-AP (same clone as 14099-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ASAP3 antibody (14099-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14099-1-PBS in a different storage buffer formulation.

ASAP3 Polyclonal antibody, PBS Only

ASAP3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 14099-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Development and differentiation-enhancing factor-like 1, DDEFL1, CENTB6, ASAP 3, Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14099-1-PBS targets ASAP3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5231

Product name: Recombinant human ASAP3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 558-903 aa of BC060786

Sequence: RDLLSVLEAFANGQDFGQPLPGPDAQAPEELVLHLAVKVANQASLPLVDFIIQNGGHLDAKAADGNTALHYAALYNQPDCLKLLLKGRALVGTVNEAGETALDIARKKHHKECEELLEQAQAGTFAFPLHVDYSWVISTEPGSDSEEDEEEKRCLLKLPAQAHWASGRLDISNKTYETVASLGAATPQGESEDCPPPLPVKNSSRTLVQGCARHASGDRSEVSSLSSEAPETPESLGSPASSSSLMSPLEPGDPSQAPPNSEEGLREPPGTSRPSLTSGTTPSEMYLPVRFSSESTRSYRRGARSPEDGPSARQPLPRRNVPVGITEGDGSRTGSLPASSVQLLQD

相同性解析による交差性が予測される生物種
Full Name ArfGAP with SH3 domain, ankyrin repeat and PH domain 3
Calculated molecular weight 903 aa, 99 kDa
Observed molecular weight100 kDa
GenBank accession numberBC060786
Gene Symbol ASAP3
Gene ID (NCBI) 55616
RRIDAB_2059021
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TDY4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ArfGAP with SH3 domain, ankyrin repeat, and PH domain 3 (ASAP3) is a specific ARF6 GTPase-activating protein (GAP), also termed DDEFL1 and ACAP4, which comprises ArfGAP and ankyrin repeat (ANK) domains in the C-terminus and BAR and PH domains in the N-terminus.ASAP3 is considered to be an important regulator in cancer cell migration/invasion and actin-based cytoskeletal remodeling.

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