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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ASAP3 Polyclonal antibody (14099-1-AP)

    WB analysis using 14099-1-AP (same clone as 14099-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14099-1-AP (ASAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14099-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using ASAP3 Polyclonal antibody (14099-1-AP)

    IHC staining of mouse lung using 14099-1-AP (same clone as 14099-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 14099-1-AP (ASAP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14099-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using ASAP3 Polyclonal antibody (14099-1-AP)

    IHC staining of mouse lung using 14099-1-AP (same clone as 14099-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 14099-1-AP (ASAP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14099-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ASAP3 Polyclonal antibody (14099-1-AP)

    IF Staining of HepG2 using 14099-1-AP (same clone as 14099-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ASAP3 antibody (14099-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14099-1-PBS in a different storage buffer formulation.

ASAP3 Polyclonal antibody, PBS Only

ASAP3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 14099-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Development and differentiation-enhancing factor-like 1, DDEFL1, CENTB6, ASAP 3, Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14099-1-PBS targets ASAP3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5231

Product name: Recombinant human ASAP3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 558-903 aa of BC060786

Sequence: RDLLSVLEAFANGQDFGQPLPGPDAQAPEELVLHLAVKVANQASLPLVDFIIQNGGHLDAKAADGNTALHYAALYNQPDCLKLLLKGRALVGTVNEAGETALDIARKKHHKECEELLEQAQAGTFAFPLHVDYSWVISTEPGSDSEEDEEEKRCLLKLPAQAHWASGRLDISNKTYETVASLGAATPQGESEDCPPPLPVKNSSRTLVQGCARHASGDRSEVSSLSSEAPETPESLGSPASSSSLMSPLEPGDPSQAPPNSEEGLREPPGTSRPSLTSGTTPSEMYLPVRFSSESTRSYRRGARSPEDGPSARQPLPRRNVPVGITEGDGSRTGSLPASSVQLLQD

相同性解析による交差性が予測される生物種
Full Name ArfGAP with SH3 domain, ankyrin repeat and PH domain 3
Calculated molecular weight 903 aa, 99 kDa
Observed molecular weight100 kDa
GenBank accession numberBC060786
Gene Symbol ASAP3
Gene ID (NCBI) 55616
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TDY4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ArfGAP with SH3 domain, ankyrin repeat, and PH domain 3 (ASAP3) is a specific ARF6 GTPase-activating protein (GAP), also termed DDEFL1 and ACAP4, which comprises ArfGAP and ankyrin repeat (ANK) domains in the C-terminus and BAR and PH domains in the N-terminus.ASAP3 is considered to be an important regulator in cancer cell migration/invasion and actin-based cytoskeletal remodeling.

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