Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, L02 cells |
| Positive IHC detected in | mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 2 publications below |
Product Information
14099-1-AP targets ASAP3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5231 Product name: Recombinant human ASAP3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 558-903 aa of BC060786 Sequence: RDLLSVLEAFANGQDFGQPLPGPDAQAPEELVLHLAVKVANQASLPLVDFIIQNGGHLDAKAADGNTALHYAALYNQPDCLKLLLKGRALVGTVNEAGETALDIARKKHHKECEELLEQAQAGTFAFPLHVDYSWVISTEPGSDSEEDEEEKRCLLKLPAQAHWASGRLDISNKTYETVASLGAATPQGESEDCPPPLPVKNSSRTLVQGCARHASGDRSEVSSLSSEAPETPESLGSPASSSSLMSPLEPGDPSQAPPNSEEGLREPPGTSRPSLTSGTTPSEMYLPVRFSSESTRSYRRGARSPEDGPSARQPLPRRNVPVGITEGDGSRTGSLPASSVQLLQD 相同性解析による交差性が予測される生物種 |
| Full Name | ArfGAP with SH3 domain, ankyrin repeat and PH domain 3 |
| Calculated molecular weight | 903 aa, 99 kDa |
| Observed molecular weight | 100 kDa |
| GenBank accession number | BC060786 |
| Gene Symbol | ASAP3 |
| Gene ID (NCBI) | 55616 |
| RRID | AB_2059021 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TDY4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ArfGAP with SH3 domain, ankyrin repeat, and PH domain 3 (ASAP3) is a specific ARF6 GTPase-activating protein (GAP), also termed DDEFL1 and ACAP4, which comprises ArfGAP and ankyrin repeat (ANK) domains in the C-terminus and BAR and PH domains in the N-terminus.ASAP3 is considered to be an important regulator in cancer cell migration/invasion and actin-based cytoskeletal remodeling.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ASAP3 antibody 14099-1-AP | Download protocol |
| IHC protocol for ASAP3 antibody 14099-1-AP | Download protocol |
| WB protocol for ASAP3 antibody 14099-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Aging (Albany NY) Bone morphogenetic protein 4 (BMP4) alleviates hepatic steatosis by increasing hepatic lipid turnover and inhibiting the mTORC1 signaling axis in hepatocytes. | ||
J Cell Mol Med Long non-coding RNA (lncRNA) H19 induces hepatic steatosis through activating MLXIPL and mTORC1 networks in hepatocytes. |
