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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated ARPC2 Monoclonal antibody

ARPC2 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D8E3

Cat no : CL488-68145

Synonyms

ARC34, Arp2/3 complex 34 kDa subunit, ARPC2, p34 Arc, PNAS 139, PRO2446

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL488-68145

    IF Staining of HeLa using CL488-68145

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 ARPC2 antibody (CL488-68145, Clone: 1D8E3 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-68145

    FC experiment of HeLa using CL488-68145

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human ARPC2 (CL488-68145, Clone:1D8E3) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ARPC2 Ready-To-Use IHC Kit. ARPC2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68145-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-68145 targets ARPC2 in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ARPC2 fusion protein Ag7082 相同性解析による交差性が予測される生物種
Full Name actin related protein 2/3 complex, subunit 2, 34kDa
Calculated molecular weight 34 kDa
Observed molecular weight34 kDa
GenBank accession numberBC000590
Gene symbol ARPC2
Gene ID (NCBI) 10109
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ARPC2 antibody CL488-68145Download protocol
FC protocol for CL Plus 488 ARPC2 antibody CL488-68145Download protocol
Standard Protocols
Click here to view our Standard Protocols