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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated ARNT,HIF1B Monoclonal anti (CL594-66732)

    IF Staining of HepG2 using CL594-66732

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594-conjugated ARNT,HIF1B antibody (CL594-66732, Clone: 3B12C5 ) at dilution of 1:200, CL488-Phalloidin (green).

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated ARNT,HIF1B Monoclonal anti (CL594-66732)

    FC experiment of HeLa using CL594-66732

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human ARNT,HIF1B (CL594-66732, Clone:3B12C5) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite®594-conjugated ARNT,HIF1B Monoclonal antibody

ARNT,HIF1B Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-66732
Clone No.3B12C5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

ARNT/HIF-1 beta, ARNT, HIF-1 beta, HIF1B, HIF 1 beta

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66732 targets ARNT,HIF1B in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag5507

Product name: Recombinant human ARNT,HIF1B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-348 aa of BC060838

Sequence: MAATTANPEMTSDVPSLGPAIASGNSGPGIQGGGAIVQRAIKRRPGLDFDDDGEGNSKFLRCDDDQMSNDKERFARSDDEQSSADKERLARENHSEIERRRRNKMTAYITELSDMVPTCSALARKPDKLTILRMAVSHMKSLRGTGNTSTDGSYKPSFLTDQELKHLILEAADGFLFIVSCETGRVVYVSDSVTPVLNQQQSEWFGSTLYDQVHPDDVDKLREQLSTSENALTGRILDLKTGTVKKEGQQSSMRMCMGSRRSFICRMRCGSSSVDPVSVNRLSFVRNRCRNGLGSVKDGEPHFVVVHCTGYIKAWPPAGVSLPDDDPEAGQGSKFCLVAIGRLQVTSS

相同性解析による交差性が予測される生物種
Full Name aryl hydrocarbon receptor nuclear translocator
Calculated molecular weight 416 aa, 46 kDa, 87 kDa
Observed molecular weight 87 kDa
GenBank accession numberBC060838
Gene Symbol ARNT/HIF-1 beta
Gene ID (NCBI) 405
RRIDAB_2883619
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP27540
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HIF1B, also named as ARNT, is a 789 amino acid protein, which forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. HIF1B is Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia

Protocols

Product Specific Protocols
IF protocol for CL594 ARNT,HIF1B antibody CL594-66732Download protocol
FC protocol for CL594 ARNT,HIF1B antibody CL594-66732Download protocol
Standard Protocols
Click here to view our Standard Protocols