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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ARL3 Monoclonal antibody (68421-1-Ig)

    WB analysis of HeLa using 68421-1-Ig (same clone as 68421-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 68421-1-Ig (ARL3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68421-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rabbit testis tissue using ARL3 Monoclonal antibody (68421-1-Ig)

    WB analysis of rabbit testis using 68421-1-Ig (same clone as 68421-1-PBS)

    rabbit testis tissue were subjected to SDS PAGE followed by western blot with 68421-1-Ig (ARL3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68421-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat retina tissue using ARL3 Monoclonal antibody (68421-1-Ig)

    WB analysis of rat retina using 68421-1-Ig (same clone as 68421-1-PBS)

    rat retina tissue were subjected to SDS PAGE followed by western blot with 68421-1-Ig (ARL3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68421-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using ARL3 Monoclonal antibody (68421-1-Ig)

    IF Staining of hTERT-RPE1 using 68421-1-Ig (same clone as 68421-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using ARL3 antibody (68421-1-Ig, Clone: 2E5B12 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68421-1-PBS in a different storage buffer formulation.

ARL3 Monoclonal antibody, PBS Only

ARL3 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68421-1-PBS
Clone No.2E5B12

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, rabbit

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

ADP ribosylation factor like 3, ARFL3, ARL3

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68421-1-PBS targets ARL3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, rat, rabbit samples.

Tested Reactivity Human, rat, rabbit
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag21387

Product name: Recombinant human ARL3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-182 aa of BC009841

Sequence: MGLLSILRKLKSAPDQEVRILLLGLDNAGKTTLLKQLASEDISHITPTQGFNIKSVQSQGFKLNVWDIGGQRKIRPYWKNYFENTDILIYVIDSADRKRFEETGQELAELLEEEKLSCVPVLIFANKQDLLTAAPASEIAEGLNLHTIRDRVWQIQSCSALTGEGVQDGMNWVCKNVNAKKK

相同性解析による交差性が予測される生物種
Full Name ADP-ribosylation factor-like 3
Calculated molecular weight 20 kDa
Observed molecular weight 20-24 kDa
GenBank accession numberBC009841
Gene Symbol ARL3
Gene ID (NCBI) 403
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP36405
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ARL3 (ADP-ribosylation factor-like 3) is a member of the ADP-ribosylation factor family of GTP-binding proteins. ARL3 binds guanine nucleotides but lacks ADP-ribosylation factor activity. ARL3 is believed to be involved in ciliary and microtubule-dependent processes.

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