Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, rabbit testis tissue, rat retina tissue |
| Positive IF/ICC detected in | hTERT-RPE1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68421-1-Ig targets ARL3 in WB, IF/ICC, ELISA applications and shows reactivity with Human, rat, rabbit samples.
| Tested Reactivity | Human, rat, rabbit |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21387 Product name: Recombinant human ARL3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-182 aa of BC009841 Sequence: MGLLSILRKLKSAPDQEVRILLLGLDNAGKTTLLKQLASEDISHITPTQGFNIKSVQSQGFKLNVWDIGGQRKIRPYWKNYFENTDILIYVIDSADRKRFEETGQELAELLEEEKLSCVPVLIFANKQDLLTAAPASEIAEGLNLHTIRDRVWQIQSCSALTGEGVQDGMNWVCKNVNAKKK 相同性解析による交差性が予測される生物種 |
| Full Name | ADP-ribosylation factor-like 3 |
| Calculated molecular weight | 20 kDa |
| Observed molecular weight | 20-24 kDa |
| GenBank accession number | BC009841 |
| Gene Symbol | ARL3 |
| Gene ID (NCBI) | 403 |
| RRID | AB_3085136 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P36405 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ARL3 (ADP-ribosylation factor-like 3) is a member of the ADP-ribosylation factor family of GTP-binding proteins. ARL3 binds guanine nucleotides but lacks ADP-ribosylation factor activity. ARL3 is believed to be involved in ciliary and microtubule-dependent processes.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ARL3 antibody 68421-1-Ig | Download protocol |
| WB protocol for ARL3 antibody 68421-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
