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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human colon tissue using ARHGEF11 Recombinant antibody (84149-1-RR)

    IHC staining of human colon using 84149-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84149-1-RR (ARHGEF11 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using ARHGEF11 Recombinant antibody (84149-1-RR)

    IHC staining of human colon using 84149-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84149-1-RR (ARHGEF11 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HuH-7 cells using ARHGEF11 Recombinant antibody (84149-1-RR)

    IF Staining of HuH-7 using 84149-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HuH-7 cells using ARHGEF11 antibody (84149-1-RR, Clone: 241427A4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84149-1-RR

    Affinity and Kinetic Characterization of 84149-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84149-1-RR against Human ARHGEF11 were performed. The affinity constant is 1.09 nM.

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ARHGEF11 Recombinant antibody

ARHGEF11 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 84149-1-RR
Clone No.241427A4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Synonyms

241427A4, GTRAP48, KIAA0380, PDZ RHOGEF

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHuH-7 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84149-1-RR targets ARHGEF11 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ARHGEF11 fusion protein Ag33104 相同性解析による交差性が予測される生物種
Full Name Rho guanine nucleotide exchange factor (GEF) 11
Calculated molecular weight168 kDa
Gene Symbol ARHGEF11
Gene ID (NCBI) 9826
RRIDAB_3671713
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO15085
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARHGEF11 (Rho Guanine Nucleotide Exchange Factor 11), also known as PDZ-RhoGEF or GTRAP48. ARHGEF11 contains several functional domains, including a PDZ domain and a RhoGEF domain. The PDZ domain allows it to interact with various proteins, such as ZO-1 and plexins, while the RhoGEF domain enables it to activate Rho GTPases. ARHGEF11 is a multifunctional protein that plays crucial roles in cell adhesion, signal transduction, and development. Its dysregulation has been linked to several diseases, making it an important area of research for potential therapeutic applications.

Protocols

Product Specific Protocols
IHC protocol for ARHGEF11 antibody 84149-1-RRDownload protocol
IF protocol for ARHGEF11 antibody 84149-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols