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Validation Data Gallery

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  • Sandwich ELISA standard curve of MP01063-2

    Sandwich ELISA standard curve of MP01063-2

    Sandwich ELISA standard curve of MP01063-2, Human ARHGEF11 Recombinant Matched Antibody Pair - PBS only. 84149-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag33104. 84149-1-PBS was HRP conjugated as the detection antibody. Range: 156-5000 pg/mL.

  • Sample test of MP01063-2

    Sample test of MP01063-2

    The mean ARHGEF11 concentration was determined to be 4.0 ng/mL in HEK-293T cell extract based on a 3.2 mg/mL extract load.

  • Immunohistochemistry (IHC) staining of human colon tissue using ARHGEF11 Recombinant antibody (84149-1-RR)

    IHC staining of human colon using 84149-1-RR (same clone as 84149-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84149-1-RR (ARHGEF11 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84149-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using ARHGEF11 Recombinant antibody (84149-1-RR)

    IHC staining of human colon using 84149-1-RR (same clone as 84149-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84149-1-RR (ARHGEF11 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84149-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HuH-7 cells using ARHGEF11 Recombinant antibody (84149-1-RR)

    IF Staining of HuH-7 using 84149-1-RR (same clone as 84149-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HuH-7 cells using ARHGEF11 antibody (84149-1-RR, Clone: 241427A4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84149-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01063-1

    Cytometric bead array standard curve of MP01063-1

    Cytometric bead array standard curve of MP01063-1, ARHGEF11 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84149-2-PBS. Detection antibody: 84149-1-PBS. Standard: Ag33104. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP01063-2

    Cytometric bead array standard curve of MP01063-2

    Cytometric bead array standard curve of MP01063-2, ARHGEF11 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84149-3-PBS. Detection antibody: 84149-1-PBS. Standard: Ag33104. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84149-1-PBS

    Affinity and Kinetic Characterization of 84149-1-PBS

    Biolayer interferometry (BLl) kinetic assay of 84149-1-PBS against Human ARHGEF11 was performed. The affinity constant is below 1 pM.

  • Affinity and Kinetic Characterization of 84149-1-RR

    Affinity and Kinetic Characterization of 84149-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84149-1-RR against Human ARHGEF11 were performed. The affinity constant is 1.09 nM.

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ARHGEF11 Recombinant antibody, PBS Only (Detector)

ARHGEF11 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84149-1-PBS
Clone No.241427A4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

241427A4, GTRAP48, KIAA0380, PDZ RHOGEF

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84149-1-PBS targets ARHGEF11 as part of a matched antibody pair:

MP01063-1: 84149-2-PBS capture and 84149-1-PBS detection (validated in Cytometric bead array)

MP01063-2: 84149-3-PBS capture and 84149-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ARHGEF11 fusion protein Ag33104 相同性解析による交差性が予測される生物種
Full Name Rho guanine nucleotide exchange factor (GEF) 11
Calculated molecular weight168 kDa
Gene Symbol ARHGEF11
Gene ID (NCBI) 9826
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO15085
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ARHGEF11 (Rho Guanine Nucleotide Exchange Factor 11), also known as PDZ-RhoGEF or GTRAP48. ARHGEF11 contains several functional domains, including a PDZ domain and a RhoGEF domain. The PDZ domain allows it to interact with various proteins, such as ZO-1 and plexins, while the RhoGEF domain enables it to activate Rho GTPases. ARHGEF11 is a multifunctional protein that plays crucial roles in cell adhesion, signal transduction, and development. Its dysregulation has been linked to several diseases, making it an important area of research for potential therapeutic applications.