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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARHGAP44 Polyclonal antibody (32382-1-AP)

    WB analysis using 32382-1-AP (same clone as 32382-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 32382-1-AP (ARHGAP44 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32382-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ARHGAP44 Polyclonal antibody (32382-1-AP)

    WB analysis using 32382-1-AP (same clone as 32382-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 32382-1-AP (ARHGAP44 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32382-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using ARHGAP44 Polyclonal antibody (32382-1-AP)

    IHC staining of human urothelial carcinoma using 32382-1-AP (same clone as 32382-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 32382-1-AP (ARHGAP44 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32382-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using ARHGAP44 Polyclonal antibody (32382-1-AP)

    IHC staining of human urothelial carcinoma using 32382-1-AP (same clone as 32382-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 32382-1-AP (ARHGAP44 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32382-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ARHGAP44 Polyclonal antibody (32382-1-AP)

    IF Staining of HEK-293 using 32382-1-AP (same clone as 32382-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ARHGAP44 antibody (32382-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 32382-1-PBS in a different storage buffer formulation.

ARHGAP44 Polyclonal antibody, PBS Only

ARHGAP44 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 32382-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

KIAA0672, NPC-A-10, Rho GTPase Activating Protein 44, Rho GTPase-activating protein 44, Rho GTPase-Activating Protein RICH2

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

32382-1-PBS targets ARHGAP44 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag38196

Product name: Recombinant human ARHGAP44 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 580-772 aa of NM_001321166

Sequence: LQPGPERTSTTKSKELSPGSAQKGSPGSSQGTACAGTQPGAQPGAQPGASPSPSQPPADQSPHTLRKVSKKLAPIPPKVPFGQPGAMADQSAGQPSPVSLSPTPPSTPSPYGLSYPQGYSLASGQLSPAAAPPLASPSVFTSTLSKSRPTPKPRQRPTLPPPQPPTVNLSASSPQSTEAPMLDGMSPGESMST

相同性解析による交差性が予測される生物種
Full Name Rho-type GTPase-activating protein RICH2
Calculated molecular weight818aa, 89 kDa
Observed molecular weight80-100 kDa
GenBank accession numberNM_001321166
Gene Symbol RICH2
Gene ID (NCBI) 9912
RRIDAB_3742591
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ17R89
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Rho GTPase-activating protein 44 (ARHGAP44) belongs to the Rho GTPase-activating protein (RhoGAP) family, also known as Rich2.ARHGAP44 plays an important role in cytoskeletal dynamics, cellular motility and signaling. Its high expression in osteosarcoma correlates with tumor malignancy, suggesting its potential as a potential therapeutic target. In addition, ARHGAP44 may also be involved in the pathogenesis of diabetes and cardiovascular diseases, and its specific role in these diseases needs to be further investigated in the future.

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