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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARHGAP44 Polyclonal antibody (32382-1-AP)

    WB analysis using 32382-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32382-1-AP (ARHGAP44 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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ARHGAP44 Polyclonal antibody

ARHGAP44 Polyclonal Antibody for WB, ELISA
Cat No. 32382-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

RhoGAP interacting with CIP4 homologs protein 2, Rho GTPase-Activating Protein RICH2, Rho GTPase-activating protein 44, Rho GTPase Activating Protein 44, NPC-A-10

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, LNCaP cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32382-1-AP targets ARHGAP44 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARHGAP44 fusion protein Ag38196 相同性解析による交差性が予測される生物種
Full Name Rho-type GTPase-activating protein RICH2
Calculated molecular weight818aa, 89 kDa
Observed molecular weight80-100 kDa
GenBank accession numberNM_001321166
Gene Symbol RICH2
Gene ID (NCBI) 9912
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ17R89
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rho GTPase-activating protein 44 (ARHGAP44) belongs to the Rho GTPase-activating protein (RhoGAP) family, also known as Rich2.ARHGAP44 plays an important role in cytoskeletal dynamics, cellular motility and signaling. Its high expression in osteosarcoma correlates with tumor malignancy, suggesting its potential as a potential therapeutic target. In addition, ARHGAP44 may also be involved in the pathogenesis of diabetes and cardiovascular diseases, and its specific role in these diseases needs to be further investigated in the future.

Protocols

Product Specific Protocols
WB protocol for ARHGAP44 antibody 32382-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols