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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using ARHGAP12 Polyclonal antibody (17855-1-AP)

    IHC staining of mouse cerebellum using 17855-1-AP (same clone as 17855-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 17855-1-AP (ARHGAP12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17855-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ARHGAP12 Polyclonal antibody (17855-1-AP)

    IHC staining of mouse kidney using 17855-1-AP (same clone as 17855-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17855-1-AP (ARHGAP12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17855-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ARHGAP12 Polyclonal antibody (17855-1-AP)

    IHC staining of human kidney using 17855-1-AP (same clone as 17855-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17855-1-AP (ARHGAP12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17855-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ARHGAP12 Polyclonal antibody (17855-1-AP)

    IHC staining of human kidney using 17855-1-AP (same clone as 17855-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17855-1-AP (ARHGAP12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17855-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using ARHGAP12 Polyclonal antibody (17855-1-AP)

    IF Staining of mouse cerebellum using 17855-1-AP (same clone as 17855-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using ARHGAP12 antibody (17855-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17855-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat kidney tissue using ARHGAP12 Polyclonal antibody (17855-1-AP)

    IF Staining of rat kidney using 17855-1-AP (same clone as 17855-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat kidney tissue using ARHGAP12 antibody (17855-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17855-1-PBS in a different storage buffer formulation.

ARHGAP12 Polyclonal antibody, PBS Only

ARHGAP12 Polyclonal Antibody for IHC, IF-P, Indirect ELISA
Cat No. 17855-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF-P, Indirect ELISA

Synonyms

Rho GTPase-activating protein 12, Rho-type GTPase-activating protein 12

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17855-1-PBS targets ARHGAP12 in IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12309

Product name: Recombinant human ARHGAP12 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 486-841 aa of BC094719

Sequence: AVLQGSSLLFTKTQGSSTSWFGSNQSKPEFTVDLKGATIEMASKDKSSKKNVFELKTRQGTELLIQSDNDTVINDWFKVLSSTINNQAVETDEGIEEEIPDSPGIEKHDKEKEQKDPKKLRSFKVSSIDSSEQKKTKKNLKKFLTRRPTLQAVREKGYIKDQVFGSNLANLCQRENGTVPKFVKLCIEHVEEHGLDIDGIYRVSGNLAVIQKLRFAVNHDEKLDLNDSKWEDIHVITGALKMFFRELPEPLFTFNHFNDFVNAIKQEPRQRVAAVKDLIRQLPKPNQDTMQILFRHLRRVIENGEKNRMTYQSIAIVFGPTLLKPEKETGNIAVHTVYQNQIVELILLELSSIFGR

相同性解析による交差性が予測される生物種
Full Name Rho GTPase activating protein 12
Calculated molecular weight 841 aa, 96 kDa
GenBank accession numberBC094719
Gene Symbol ARHGAP12
Gene ID (NCBI) 94134
RRIDAB_3741937
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IWW6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ARHGAP12 belongs to the Rho GTPase-activating protein (RhoGAP) family. RhoGAP is a key regulatory factor of the small GTPase Rho family and serves as a molecular switch that regulates various cellular processes. ARHGAP12 is involved in suppressing tumor formation by regulating cell invasion and adhesion.

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