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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using ARHGAP12 Polyclonal antibody (17855-1-AP)

    IHC staining of mouse cerebellum using 17855-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 17855-1-AP (ARHGAP12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ARHGAP12 Polyclonal antibody (17855-1-AP)

    IHC staining of mouse kidney using 17855-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17855-1-AP (ARHGAP12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ARHGAP12 Polyclonal antibody (17855-1-AP)

    IHC staining of human kidney using 17855-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17855-1-AP (ARHGAP12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ARHGAP12 Polyclonal antibody (17855-1-AP)

    IHC staining of human kidney using 17855-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17855-1-AP (ARHGAP12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ARHGAP12 Polyclonal antibody

ARHGAP12 Polyclonal Antibody for IHC, ELISA
Cat No. 17855-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Synonyms

Rho GTPase-activating protein 12, Rho-type GTPase-activating protein 12

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman kidney tissue, mouse cerebellum tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17855-1-AP targets ARHGAP12 in IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12309

Product name: Recombinant human ARHGAP12 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 486-841 aa of BC094719

Sequence: AVLQGSSLLFTKTQGSSTSWFGSNQSKPEFTVDLKGATIEMASKDKSSKKNVFELKTRQGTELLIQSDNDTVINDWFKVLSSTINNQAVETDEGIEEEIPDSPGIEKHDKEKEQKDPKKLRSFKVSSIDSSEQKKTKKNLKKFLTRRPTLQAVREKGYIKDQVFGSNLANLCQRENGTVPKFVKLCIEHVEEHGLDIDGIYRVSGNLAVIQKLRFAVNHDEKLDLNDSKWEDIHVITGALKMFFRELPEPLFTFNHFNDFVNAIKQEPRQRVAAVKDLIRQLPKPNQDTMQILFRHLRRVIENGEKNRMTYQSIAIVFGPTLLKPEKETGNIAVHTVYQNQIVELILLELSSIFGR

相同性解析による交差性が予測される生物種
Full Name Rho GTPase activating protein 12
Calculated molecular weight 841 aa, 96 kDa
GenBank accession numberBC094719
Gene Symbol ARHGAP12
Gene ID (NCBI) 94134
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IWW6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARHGAP12 belongs to the Rho GTPase-activating protein (RhoGAP) family. RhoGAP is a key regulatory factor of the small GTPase Rho family and serves as a molecular switch that regulates various cellular processes. ARHGAP12 is involved in suppressing tumor formation by regulating cell invasion and adhesion.

Protocols

Product Specific Protocols
IHC protocol for ARHGAP12 antibody 17855-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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