Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 16504-1-AP (same clone as 16504-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 16504-1-AP (ARH3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16504-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 16504-1-AP (same clone as 16504-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 16504-1-AP (ARH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16504-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 16504-1-AP (same clone as 16504-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 16504-1-AP (ARH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16504-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 16504-1-AP (same clone as 16504-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 16504-1-AP (ARH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16504-1-PBS in a different storage buffer formulation.
WB analysis of mouse heart using 16504-1-AP (same clone as 16504-1-PBS)
mouse heart tissue were subjected to SDS PAGE followed by western blot with 16504-1-AP (ARH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16504-1-PBS in a different storage buffer formulation.
WB analysis of mouse lung using 16504-1-AP (same clone as 16504-1-PBS)
mouse lung tissue were subjected to SDS PAGE followed by western blot with 16504-1-AP (ARH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16504-1-PBS in a different storage buffer formulation.
WB analysis of mouse kidney using 16504-1-AP (same clone as 16504-1-PBS)
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16504-1-AP (ARH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16504-1-PBS in a different storage buffer formulation.
WB analysis of mouse thymus using 16504-1-AP (same clone as 16504-1-PBS)
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 16504-1-AP (ARH3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16504-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 16504-1-AP (same clone as 16504-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16504-1-AP (ARH3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16504-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 16504-1-AP (same clone as 16504-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16504-1-AP (ARH3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16504-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 16504-1-AP (same clone as 16504-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16504-1-AP (ARH3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16504-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 16504-1-AP (same clone as 16504-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16504-1-AP (ARH3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16504-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 16504-1-AP (same clone as 16504-1-PBS)
Immunofluorescent analysis of HepG2 cells using 16504-1-AP (ARH3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16504-1-PBS in a different storage buffer formulation.

ARH3 Polyclonal antibody, PBS Only

ARH3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 16504-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16504-1-PBS targets ARH3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9683 Product name: Recombinant human ADPRHL2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-363 aa of BC014169 Sequence: MAAAAMAAAAGGGAGAARSLSRFRGCLAGALLGDCVGSFYEAHDTVDLTSVLRHVQSLEPDPGTPGSERTEALYYTDDTAMARALVQSLLAKEAFDEVDMAHRFAQEYKKDPDRGYGAGVVTVFKKLLNPKCRDVFEPARAQFNGKGSYGNGGAMRVAGISLAYSSVQDVQKFARLSAQLTHASSLGYNGAILQALAVHLALQGESSSEHFLKQLLGHMEDLEGDAQSVLDARELGMEERPYSSRLKKIGELLDQASVTREEVVSELGNGIAAFESVPTAIYCFLRCMEPDPEIPSAFNSLQRTLIYSISLGGDTDTIATMAGAIAGAYYGMDQVPESWQQSCEGYEETDILAQSLHRVFQKS 相同性解析による交差性が予測される生物種
Full Name	ADP-ribosylhydrolase like 2
Calculated molecular weight	363 aa, 39 kDa
Observed molecular weight	39 kDa
GenBank accession number	BC014169
Gene Symbol	ARH3
Gene ID (NCBI)	54936
RRID	AB_2224919
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NX46
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

ARH3 can be detected as two bands between 39 and 40 kDa.(PMID: 16278211/32636369)

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 16504-1-AP (same clone as 16504-1-PBS)

WB analysis of HeLa using 16504-1-AP (same clone as 16504-1-PBS)

WB analysis of mouse brain using 16504-1-AP (same clone as 16504-1-PBS)

WB analysis of HepG2 using 16504-1-AP (same clone as 16504-1-PBS)

WB analysis of mouse heart using 16504-1-AP (same clone as 16504-1-PBS)

WB analysis of mouse lung using 16504-1-AP (same clone as 16504-1-PBS)

WB analysis of mouse kidney using 16504-1-AP (same clone as 16504-1-PBS)

WB analysis of mouse thymus using 16504-1-AP (same clone as 16504-1-PBS)

IHC staining of mouse testis using 16504-1-AP (same clone as 16504-1-PBS)

IHC staining of mouse testis using 16504-1-AP (same clone as 16504-1-PBS)

IHC staining of mouse brain using 16504-1-AP (same clone as 16504-1-PBS)