Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 16504-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16504-1-AP (ARH3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 16504-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 16504-1-AP (ARH3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 16504-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16504-1-AP (ARH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 16504-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 16504-1-AP (ARH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 16504-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 16504-1-AP (ARH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 16504-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16504-1-AP (ARH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16504-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16504-1-AP (ARH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 16504-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 16504-1-AP (ARH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of mouse testis using 16504-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16504-1-AP (ARH3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 16504-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16504-1-AP (ARH3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 16504-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16504-1-AP (ARH3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 16504-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16504-1-AP (ARH3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 16504-1-AP
Immunofluorescent analysis of HepG2 cells using 16504-1-AP (ARH3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

ARH3 Polyclonal antibody

ARH3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 16504-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

ADP-ribosylhydrolase 3, ADP-ribose glycohydrolase ARH3, ADPRHL2, ADPRHL 2, [Protein ADP-ribosylserine] hydrolase

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse brain tissue, HeLa cells, HepG2 cells, mouse heart tissue, mouse lung tissue, mouse kidney tissue, mouse thymus tissue, rat brain tissue
Positive IHC detected in	mouse testis tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IP	See 1 publications below

Product Information

16504-1-AP targets ARH3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9683 Product name: Recombinant human ADPRHL2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-363 aa of BC014169 Sequence: MAAAAMAAAAGGGAGAARSLSRFRGCLAGALLGDCVGSFYEAHDTVDLTSVLRHVQSLEPDPGTPGSERTEALYYTDDTAMARALVQSLLAKEAFDEVDMAHRFAQEYKKDPDRGYGAGVVTVFKKLLNPKCRDVFEPARAQFNGKGSYGNGGAMRVAGISLAYSSVQDVQKFARLSAQLTHASSLGYNGAILQALAVHLALQGESSSEHFLKQLLGHMEDLEGDAQSVLDARELGMEERPYSSRLKKIGELLDQASVTREEVVSELGNGIAAFESVPTAIYCFLRCMEPDPEIPSAFNSLQRTLIYSISLGGDTDTIATMAGAIAGAYYGMDQVPESWQQSCEGYEETDILAQSLHRVFQKS 相同性解析による交差性が予測される生物種
Full Name	ADP-ribosylhydrolase like 2
Calculated molecular weight	363 aa, 39 kDa
Observed molecular weight	39 kDa
GenBank accession number	BC014169
Gene Symbol	ARH3
Gene ID (NCBI)	54936
RRID	AB_2224919
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NX46
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ARH3 can be detected as two bands between 39 and 40 kDa.(PMID: 16278211/32636369)

Protocols

Product Specific Protocols
IF protocol for ARH3 antibody 16504-1-AP	Download protocol
IHC protocol for ARH3 antibody 16504-1-AP	Download protocol
WB protocol for ARH3 antibody 16504-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,rat	WB,IP	Nat Commun Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration. Authors - Megha Jhanji View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 16504-1-AP

WB analysis of mouse thymus using 16504-1-AP

WB analysis of HepG2 using 16504-1-AP

WB analysis of mouse heart using 16504-1-AP

WB analysis of mouse lung using 16504-1-AP

WB analysis of mouse kidney using 16504-1-AP

WB analysis of HeLa using 16504-1-AP

WB analysis of mouse brain using 16504-1-AP

IHC staining of mouse testis using 16504-1-AP