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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AQP9 Polyclonal antibody (20380-1-AP)

    WB analysis using 20380-1-AP (same clone as 20380-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20380-1-AP (AQP9 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20380-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using AQP9 Polyclonal antibody (20380-1-AP)

    WB analysis using 20380-1-AP (same clone as 20380-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20380-1-AP (AQP9 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20380-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using AQP9 Polyclonal antibody (20380-1-AP)

    WB analysis using 20380-1-AP (same clone as 20380-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20380-1-AP (AQP9 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20380-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using AQP9 Polyclonal antibody (20380-1-AP)

    IHC staining of human liver using 20380-1-AP (same clone as 20380-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20380-1-AP (AQP9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20380-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using AQP9 Polyclonal antibody (20380-1-AP)

    IHC staining of human liver using 20380-1-AP (same clone as 20380-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20380-1-AP (AQP9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20380-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using AQP9 Polyclonal antibody (20380-1-AP)

    IF Staining of mouse testis using 20380-1-AP (same clone as 20380-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using AQP9 antibody (20380-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20380-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using AQP9 Polyclonal antibody (20380-1-AP)

    IF Staining of mouse brain using 20380-1-AP (same clone as 20380-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using AQP9 antibody (20380-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20380-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver tissue using AQP9 Polyclonal antibody (20380-1-AP)

    IF Staining of human liver using 20380-1-AP (same clone as 20380-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using AQP9 antibody (20380-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20380-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver tissue using AQP9 Polyclonal antibody (20380-1-AP)

    IF Staining of human liver using 20380-1-AP (same clone as 20380-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using AQP9 antibody (20380-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20380-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using AQP9 Polyclonal antibody (20380-1-AP)

    IF Staining of mouse testis using 20380-1-AP (same clone as 20380-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using AQP9 antibody (20380-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20380-1-PBS in a different storage buffer formulation.

AQP9 Polyclonal antibody, PBS Only

AQP9 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 20380-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

AQP 9, AQP-9, Aquaglyceroporin 9, Aquaglyceroporin-9, Aquaporin-9

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20380-1-PBS targets AQP9 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14200

Product name: Recombinant human AQP9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 211-295 aa of BC026258

Sequence: SGCAMNPARDLSPRLFTALAGWGFEVFRAGNNFWWIPVVGPLVGAVIGGLIYVLVIEIHHPEPDSVFKAEQSEDKPEKYELSVIM

相同性解析による交差性が予測される生物種
Full Name aquaporin 9
Calculated molecular weight 295 aa, 31 kDa
Observed molecular weight31 kDa
GenBank accession numberBC026258
Gene Symbol AQP9
Gene ID (NCBI) 366
RRIDAB_2935448
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO43315
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Aquaporins (AQPs), also known as water channel proteins, are members of a large protein family that osmotically modulate water fluid homeostasis in several tissues. AQP9 (Aquaporin 9) is a member of the aquaporin family that acts as a water-selective membrane channel, and it may be involved in cell migration, angiogenesis, and tumor growth. AQP9 is involved in arsenic uptake in hepatocellular carcinoma cells, in which p38-MAPK may play a limited role. AQP9 was demonstrated to promote astrocytoma cell invasion and motility via the AKT pathway.

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