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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AQP9 Polyclonal antibody (20380-1-AP)

    WB analysis using 20380-1-AP (same clone as 20380-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20380-1-AP (AQP9 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20380-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using AQP9 Polyclonal antibody (20380-1-AP)

    WB analysis using 20380-1-AP (same clone as 20380-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20380-1-AP (AQP9 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20380-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using AQP9 Polyclonal antibody (20380-1-AP)

    WB analysis using 20380-1-AP (same clone as 20380-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20380-1-AP (AQP9 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20380-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using AQP9 Polyclonal antibody (20380-1-AP)

    IHC staining of human liver using 20380-1-AP (same clone as 20380-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20380-1-AP (AQP9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20380-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using AQP9 Polyclonal antibody (20380-1-AP)

    IHC staining of human liver using 20380-1-AP (same clone as 20380-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20380-1-AP (AQP9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20380-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using AQP9 Polyclonal antibody (20380-1-AP)

    IF Staining of mouse testis using 20380-1-AP (same clone as 20380-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using AQP9 antibody (20380-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20380-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using AQP9 Polyclonal antibody (20380-1-AP)

    IF Staining of mouse brain using 20380-1-AP (same clone as 20380-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using AQP9 antibody (20380-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20380-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver tissue using AQP9 Polyclonal antibody (20380-1-AP)

    IF Staining of human liver using 20380-1-AP (same clone as 20380-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using AQP9 antibody (20380-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20380-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver tissue using AQP9 Polyclonal antibody (20380-1-AP)

    IF Staining of human liver using 20380-1-AP (same clone as 20380-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using AQP9 antibody (20380-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20380-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using AQP9 Polyclonal antibody (20380-1-AP)

    IF Staining of mouse testis using 20380-1-AP (same clone as 20380-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using AQP9 antibody (20380-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20380-1-PBS in a different storage buffer formulation.

AQP9 Polyclonal antibody, PBS Only

AQP9 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 20380-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

AQP 9, AQP-9, Aquaglyceroporin 9, Aquaglyceroporin-9, Aquaporin-9

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20380-1-PBS targets AQP9 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14200

Product name: Recombinant human AQP9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 211-295 aa of BC026258

Sequence: SGCAMNPARDLSPRLFTALAGWGFEVFRAGNNFWWIPVVGPLVGAVIGGLIYVLVIEIHHPEPDSVFKAEQSEDKPEKYELSVIM

相同性解析による交差性が予測される生物種
Full Name aquaporin 9
Calculated molecular weight 295 aa, 31 kDa
Observed molecular weight31 kDa
GenBank accession numberBC026258
Gene Symbol AQP9
Gene ID (NCBI) 366
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO43315
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Aquaporins (AQPs), also known as water channel proteins, are members of a large protein family that osmotically modulate water fluid homeostasis in several tissues. AQP9 (Aquaporin 9) is a member of the aquaporin family that acts as a water-selective membrane channel, and it may be involved in cell migration, angiogenesis, and tumor growth. AQP9 is involved in arsenic uptake in hepatocellular carcinoma cells, in which p38-MAPK may play a limited role. AQP9 was demonstrated to promote astrocytoma cell invasion and motility via the AKT pathway.

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