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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AQP9 Polyclonal antibody (20380-1-AP)

    WB analysis using 20380-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20380-1-AP (AQP9 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using AQP9 Polyclonal antibody (20380-1-AP)

    WB analysis using 20380-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20380-1-AP (AQP9 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using AQP9 Polyclonal antibody (20380-1-AP)

    WB analysis using 20380-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20380-1-AP (AQP9 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using AQP9 Polyclonal antibody (20380-1-AP)

    IHC staining of human liver using 20380-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20380-1-AP (AQP9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using AQP9 Polyclonal antibody (20380-1-AP)

    IHC staining of human liver using 20380-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20380-1-AP (AQP9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using AQP9 Polyclonal antibody (20380-1-AP)

    IF Staining of mouse brain using 20380-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using AQP9 antibody (20380-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using AQP9 Polyclonal antibody (20380-1-AP)

    IF Staining of mouse testis using 20380-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using AQP9 antibody (20380-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human liver tissue using AQP9 Polyclonal antibody (20380-1-AP)

    IF Staining of human liver using 20380-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using AQP9 antibody (20380-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human liver tissue using AQP9 Polyclonal antibody (20380-1-AP)

    IF Staining of human liver using 20380-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using AQP9 antibody (20380-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using AQP9 Polyclonal antibody (20380-1-AP)

    IF Staining of mouse testis using 20380-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using AQP9 antibody (20380-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

AQP9 Polyclonal antibody

AQP9 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 20380-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, CoIP, ELISA

Synonyms

AQP 9, AQP-9, Aquaglyceroporin 9, Aquaglyceroporin-9, Aquaporin-9

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inrat brain tissue, L02 cells, mouse brain tissue, mouse liver tissue, mouse lung tissue, mouse testis tissue, rat liver tissue, rat testis tissue, HuH-7 cells, U-251 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver tissue, mouse brain tissue, mouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20380-1-AP targets AQP9 in WB, IHC, IF-P, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14200

Product name: Recombinant human AQP9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 211-295 aa of BC026258

Sequence: SGCAMNPARDLSPRLFTALAGWGFEVFRAGNNFWWIPVVGPLVGAVIGGLIYVLVIEIHHPEPDSVFKAEQSEDKPEKYELSVIM

相同性解析による交差性が予測される生物種
Full Name aquaporin 9
Calculated molecular weight 295 aa, 31 kDa
Observed molecular weight31 kDa
GenBank accession numberBC026258
Gene Symbol AQP9
Gene ID (NCBI) 366
RRIDAB_2935448
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO43315
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Aquaporins (AQPs), also known as water channel proteins, are members of a large protein family that osmotically modulate water fluid homeostasis in several tissues. AQP9 (Aquaporin 9) is a member of the aquaporin family that acts as a water-selective membrane channel, and it may be involved in cell migration, angiogenesis, and tumor growth. AQP9 is involved in arsenic uptake in hepatocellular carcinoma cells, in which p38-MAPK may play a limited role. AQP9 was demonstrated to promote astrocytoma cell invasion and motility via the AKT pathway.

Protocols

Product Specific Protocols
IF protocol for AQP9 antibody 20380-1-APDownload protocol
IHC protocol for AQP9 antibody 20380-1-APDownload protocol
WB protocol for AQP9 antibody 20380-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Expert Rev Clin Immunol

AQP9 weakens the cytotoxicity of CD8+ T cells in colon adenocarcinoma by boosting M2 polarization of macrophages under hypoxia conditions

Authors - Jinping Chen
View Article
mouseWB,IHC

Int J Med Sci

Exploring the Shared Diagnostic Genes in IBD and Psoriasis through Bioinformatics and Experimental Assays

Authors - Lichun Han
View Article
humanWB,IHC,CoIP

Sci Rep

Aquaporin 9 downregulation in KRASG12V colorectal cancer and associated with increased proliferation and decreased apoptosis in cancer cells

Authors - Yankui Liu
  • KD Validated
View Article
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