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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated APOD Monoclonal antibody

APOD Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C6D10

Cat no : CL488-66215

Synonyms

Apo D, APOD, apolipoprotein D

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL488-66215

    IF Staining of HeLa using CL488-66215

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 APOD antibody (CL488-66215, Clone: 1C6D10 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-66215

    FC experiment of HepG2 using CL488-66215

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human APOD (CL488-66215, Clone:1C6D10) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of HepG2 using CL488-66215

    FC experiment of HepG2 using CL488-66215

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human APOD (CL488-66215, Clone:1C6D10) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHeLa cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66215-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-66215 targets APOD in IF, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen APOD fusion protein Ag21422 相同性解析による交差性が予測される生物種
Full Name apolipoprotein D
Calculated molecular weight 33 kDa
Observed molecular weight 30-33 kDa
GenBank accession numberBC007402
Gene symbol APOD
Gene ID (NCBI) 347
RRIDAB_2919301
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Apolipoprotein D (ApoD) is a member of the lipocalin superfamily of ligand transporters, and has been implicated in the transport of small hydrophobic molecules. ApoD is also a component of plasma high-density lipoproteins (HDL). Alteration of ApoD expression has been linked to multiple neurological disorders, including Alzheimer's disease.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 APOD antibody CL488-66215Download protocol
FC protocol for CL Plus 488 APOD antibody CL488-66215Download protocol
Standard Protocols
Click here to view our Standard Protocols