Validation Data Gallery
Tested Applications
| Positive WB detected in | fetal human brain tissue, mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87113-1-RR targets APLP1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg5634 Product name: Recombinant Human APLP1 protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 39-580 aa of NM_005166.4 Sequence: SLAGGSPGAAEAPGSAQVAGLCGRLTLHRDLRTGRWEPDPQRSRRCLRDPQRVLEYCRQMYPELQIARVEQATQAIPMERWCGGSRSGSCAHPHHQVVPFRCLPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQEAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPPPGTPDPSGTAVGDPSTRSWPPGSRVEGAEDEEEEESFPQPVDDYFVEPPQAEEEEETVPPPSSHTLAVVGKVTPTPRPTDGVDIYFGMPGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQALNEHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQADPPQAERVLLALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQMRFQVHTHLQVIEERVNQSLGLLDQNPHLAQELRPQIQELLHSEHLGPSELEAPAPGGSSEDKGGLQPPDSKDDTPMTLPKGSTEQDAASPEKEKMNPLEQYERKVNASVPRGFPFHSSEIQRDELAPAGTGVSRE 相同性解析による交差性が予測される生物種 |
| Full Name | amyloid beta (A4) precursor-like protein 1 |
| Calculated molecular weight | 72 kDa |
| Observed molecular weight | 80 kDa |
| GenBank accession number | NM_005166.4 |
| Gene Symbol | APLP1 |
| Gene ID (NCBI) | 333 |
| RRID | AB_3745348 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P51693-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
APLP1 (Amyloid Precursor-Like Protein 1) is a member of the amyloid precursor protein (APP) family, which also includes APP and APLP2. It is a type I transmembrane glycoprotein that is highly and predominantly expressed in the central nervous system, especially at the postsynaptic density of neurons. Functionally, APLP1 plays important roles in synaptic adhesion, synaptogenesis, and neurite outgrowth. It can form homophilic trans-interactions between neighboring cells, contributing to cell-cell adhesion and the formation of synaptic structures. Its proteolytic processing by α-, β-, and γ-secretases releases intracellular fragments that may participate in transcriptional regulation and signal transduction, potentially influencing gene expression and neuronal survival.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for APLP1 antibody 87113-1-RR | Download protocol |
| IHC protocol for APLP1 antibody 87113-1-RR | Download protocol |
| WB protocol for APLP1 antibody 87113-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |